A new checkerboard panel for testing bacterial markers in periodontal disease
A new checkerboard panel for testing bacterial markers in periodontal disease
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DOI：
10.1111/j.1399-302x.2005.00243.x
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发表时间：
2006-02-01
影响因子：
--
通讯作者：
Leonhardt, Å
中科院分区：
文献类型：
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作者：
Dahlén, G;Leonhardt, Å
背景/目的：多种微生物学方法已被用于检测牙周炎和牙周疾病进展的细菌标志物。大多数研究仅使用了数量有限的公认细菌种类。本研究的目的是使用“棋盘式”DNA - DNA杂交方法，评估一个新组合中的13种近期鉴定出的细菌种类与12种先前更受认可的牙周病原体（“旧组合”）之间的关联。
方法：选取50名个体，这些个体至少有一个位点探诊牙周袋深度为6mm或更深（患病）且探诊出血，以及至少有一个位点探诊牙周袋深度为3mm且探诊不出血（健康）。使用纸尖技术对每个个体的一个患病位点和一个健康位点进行取样，然后使用棋盘技术针对地高辛标记的全基因组探针对25种龈下细菌进行检测，这些细菌代表12种公认的和13种新鉴定的与牙周炎相关的细菌种类。
结果：在评分1（>10⁴）和评分3（>10⁵）时，24种（25种中的）细菌在患病位点的龈下菌斑中比在健康位点更频繁地被检测到。除了3种（中间链球菌、有害月形单胞菌和侵蚀艾肯菌）之外，旧组合中的所有细菌种类在较高评分（评分3）时都有显著差异。在新组合的细菌种类中，只有坦纳普氏菌、伴放线聚集杆菌和牙髓卟啉单胞菌在患病位点和健康位点之间显示出统计学上的显著差异。
结论：得出的结论是，坦纳普氏菌、伴放线聚集杆菌和牙髓卟啉单胞菌应被添加到用于牙周炎相关细菌菌群常规诊断的12种细菌中。
Background/aims: Various microbiological methods have been used for testing bacterial markers for periodontitis and periodontal disease progression. Most studies have used only a limited number of well recognized bacterial species. The purpose of the present study was to evaluate the association of 13 more recently identified bacterial species in a new panel in comparison with 12 previously more recognized periodontotopathogens ('old panel') using the 'checkerboard' DNA-DNA hybridization method.Methods: Fifty individuals were chosen who showed at least one site with a probing pocket depth of 6 mm or more (disease) and bleeding on probing and at least one site with a probing pocket depth of 3 mm and without bleeding on probing (health). One diseased and one healthy site on each individual were sampled with the paperpoint technique and the samples were processed in the checkerboard technique against deoxigenin-labeled whole genomic probes to 25 subgingival species representing 12 well recognized and 13 newly identified periodontitis associated species.Results: Twenty-four (out of 25) species were detected more frequently in the subgingival plaque of diseased than healthy sites both at score 1 (> 10(4)) and score 3 (> 10(5)). A significant difference at the higher score (score 3) was noticed for all species of the old panel except for three (Streptococcus intermedius, Selenomonas noxia, and Eikenella corrodens). Of the species in the new panel only Prevotella tannerae, Filifactor alocis, and Porphyromonas endodontalis showed a statistical significant difference between diseased and healthy sites.Conclusion: It was concluded that P. tannerae, F. alocis, and P. endodontalis should be added to the 12 species used for routine diagnostics of periodontitis-associated bacterial flora.