Gold-enhanced biomolecular surface imaging of cells and tissue by SIMS and MALDI mass spectrometry
Gold-enhanced biomolecular surface imaging of cells and tissue by SIMS and MALDI mass spectrometry
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DOI：
10.1021/ac0513111
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发表时间：
2006-02-01
影响因子：
7.4
通讯作者：
Piersma, SR
中科院分区：
文献类型：
--
作者：
Altelaar, AFM;Klinkert, I;Piersma, SR
等离子涂层的表面金属化增强了组织和细胞的飞行时间二次离子质谱 (TOF-SIMS) 成像中脂质和甾醇等膜成分的解吸/电离。获得了神经母细胞瘤细胞的胆固醇和其他膜成分的高分辨率图像，并揭示了亚细胞细节（分辨率1.5μm）。或者，在基质增强 SIMS 中，将 2,5-二羟基苯甲酸电喷雾到神经母细胞瘤细胞上，可以在细胞水平上对磷脂酰胆碱和鞘磷脂进行完整的分子离子成像。在斑基质辅助激光解吸/电离成像质谱中，基质涂覆的大鼠脑组织切片顶部的金沉积强烈增强了图像质量和信号强度。获得了高质量的总离子计数图像，神经肽加压素定位于第三脑室周围下丘脑区域的大鼠脑组织切片中。尽管金沉积增强信号的机制尚有争议，但我们获得的细胞和组织切片结果说明了这种样品制备技术在质谱生物分子表面成像方面的潜力。
Surface metallization by plasma coating enhances desorption/ionization of membrane components such as lipids and sterols in imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) of tissues and cells. High-resolution images of cholesterol and other membrane components were obtained for neuroblastoma cells and revealed subcellular details (resolving power 1.5 mu m). Alternatively, in matrix-enhanced SIMS, 2,5-dihydroxybenzoic acid electrosprayed on neuroblastoma cells allowed intact molecular ion imaging of phosphatidylcholine and sphingomyelin at the cellular level. Gold deposition on top of matrix-coated rat brain tissue sections strongly enhanced image quality and signal intensity in stigmatic matrix-assisted laser desorption/ionization imaging mass spectrometry. High-quality total ion count images were acquired, and the neuropeptide vasopressin was localized in the rat brain tissue section at the hypothalamic area around the third ventricle. Although the mechanism of signal enhancement by gold deposition is under debate, the results we have obtained for cells and tissue sections illustrate the potential of this sample preparation technique for biomolecular surface imaging by mass spectrometry.