High-throughput RNA in situ hybridization in mouse retina.
High-throughput RNA in situ hybridization in mouse retina.
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DOI：
10.1007/978-1-62703-080-9_15
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Blackshaw, Seth
中科院分区：
文献类型：
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作者：
Blackshaw, Seth
大规模基因表达谱研究的引入大大增加了快速获得高质量细胞表达模式的需要，这些模式被发现表现出差异表达的基因。大规模非放射性RNA原位杂交的使用使这成为可能，并极大地增加了这些数据的普遍实用性。在这里，我们描述了对小鼠视网膜切片中多达50个不同的基因特异性探针进行并行分析的协议。
The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. The use of large-scale nonradioactive RNA in situ hybridization makes this possible, and greatly increases the general usefulness of this data. Here, we describe protocols for parallel analysis of up to 50 different gene-specific probes in mouse retinal sections.