Tailoring shape and size of biogenic silver nanoparticles to enhance antimicrobial efficacy against MDR bacteria
Tailoring shape and size of biogenic silver nanoparticles to enhance antimicrobial efficacy against MDR bacteria
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DOI：
10.1016/j.micpath.2016.11.012
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发表时间：
2017-04-01
影响因子：
3.8
通讯作者：
Nautiyal, C. S.
中科院分区：
文献类型：
--
作者：
Kumari, Madhuree;Pandey, Shipra;Nautiyal, C. S.
利用绿色木霉（Trichoderma viride），通过控制pH、温度和反应时间等物理参数，以生态友好的方式合成了不同尺寸的球形、矩形、五角形和六角形银纳米粒子。采用紫外维斯光谱、动态光散射、透射电子显微镜和傅立叶变换红外光谱对纳米粒子进行了表征。观察到纳米颗粒对人类病原体的形状和尺寸依赖性抗微生物活性。发现球形纳米颗粒（2-5 nm）对宋内志贺菌、大肠杆菌、粘质沙雷氏菌、葡萄球菌显示出40、51、43、53.9和55.8%的最大抑制。金黄色葡萄球菌和铜绿假单胞菌分别显示出与对照相比32%、41%、31%、42.84%和42.80%的抑制作用，其中五边形和六边形纳米颗粒（50-100 nm）。不同几何形状和尺寸的纳米颗粒与所有测试的抗生素一起建立了针对病原体的增强的拮抗活性。用2-5 nm的球形纳米颗粒与氨苄青霉素和青霉素一起获得优异的抗菌效果。形状和尺寸在增强银纳米颗粒的抗微生物潜力方面发挥了主要作用，无论是单独的还是与抗生素协同的，抗生素可以用于对抗多药耐药病原体的传播。(C)2016由Elsevier Ltd.出版
Spherical, rectangular, penta, and hexagonal silver nanoparticles of different dimensions were biosynthesized in an eco-friendly manner by biocontrol agent, Trichoderma viride by manipulating physical parameters, pH, temperature, and reaction time. The particles were characterized by UV vis spectroscopy; Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) and Fourier Transform Infra-red Spectroscopy (FTIR). Shape and size dependent antimicrobial activity of nanoparticles against human pathogens was observed. Maximum inhibition was found with spherical nanoparticles (2-5 nm) showing 40, 51, 43, 53.9 and 55.8% against Shigella sonnei, Escherichia coli, Serratia marcescens, Staphylococcus. aureus and Pseudomonas aeruginosa respectively, where as pentagonal and hexagonal nano particles (50-100 nm) demonstrated 32, 41, 31, 42.84 and 42.80% of inhibition as compared to control. Nanoparticles of different geometry and dimension established enhanced antagonistic activity against pathogens with all the tested antibiotics. Excellent antimicrobial efficacy was obtained with spherical nanoparticles of 2-5 nm with ampicillin and penicillin. Shape and size played major role in enhancing antimicrobial potential of silver nanoparticles, both singly and synergistically with antibiotics which can be exploited to combat the spread of multidrug resistant pathogens. (C) 2016 Published by Elsevier Ltd.