Spatiotemporal dynamics of FERONIA reveal alternative endocytic pathways in response to flg22 elicitor stimuli
Spatiotemporal dynamics of FERONIA reveal alternative endocytic pathways in response to flg22 elicitor stimuli
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DOI：
10.1111/nph.18127
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发表时间：
2022-04-20
期刊：
影响因子：
9.4
通讯作者：
Luo, Xiaomin
中科院分区：
文献类型：
--
作者：
Xing, Jingjing;Ji, Dongchao;Luo, Xiaomin
植物受体样激酶FERONIA（FER）在响应多种细胞外信号中起作用，从而调节多种细胞过程，例如极化细胞生长、激素信号传导和对病原体的响应。在这里，我们报告说，在拟南芥中，鞭毛蛋白肽flg 22刺激显着促进横向流动性和解离的FER从质膜诱导协会的FER与膜微区组件。FER通过网格蛋白介导的途径以布雷菲德菌素A（BFA）敏感的方式进行组成性内吞和再循环。flg 22诱导后，FER通过两种不同的内吞途径定位于真正的内体，显示出对BFA的不同敏感性。这些结果在单粒子水平证实，FER作为一个重要的调节器在flg 22的感知和免疫激活，从而扩大了我们的理解的位置特异性蛋白质动力学和膜贩运受体/受体激酶信号。
The plant receptor-like kinase FERONIA (FER) functions in the response to multiple extracellular signals, thereby regulating diverse cellular processes, such as polarized cell growth, hormone signaling and responses to pathogens. Here, we reported that in Arabidopsis thaliana, flagellin peptide flg22 stimulus significantly promoted the lateral mobility and dissociation of FER from the plasma membrane by inducing the association of FER with membrane microdomain components. FER underwent constitutive endocytosis and recycling in a brefeldin A (BFA)-sensitive manner via a clathrin-mediated pathway. Following flg22 elicitation, FER localized to bona fide endosomes via two distinct endocytic routes, showing differential sensitivity to BFA. These results at the single-particle level confirm that FER acts as an essential regulator during flg22 perception and immune activation, thus broadening our understanding of location-specific protein dynamics and membrane trafficking in receptor/receptor kinase signaling.