Exploring the biology of lipid peroxidation-derived protein carbonylation.
Exploring the biology of lipid peroxidation-derived protein carbonylation.
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DOI：
10.1021/tx200169n
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发表时间：
2011-09-19
影响因子：
4.1
通讯作者：
Petersen DR
中科院分区：
文献类型：
--
作者：
Fritz KS;Petersen DR
活性氧和氮物质的持续过量产生导致细胞促氧化剂-抗氧化剂系统的不平衡，并且涉及许多疾病状态，包括酒精性肝病、癌症、神经障碍、炎症和心血管疾病。由持续氧化应激和脂质过氧化引起的反应性醛的积累是这些病理学发展的潜在因素。确定引起由蛋白质羰基化引起的细胞反应的生化因子仍然是开发治疗方法和改善疾病病理的关键要素。这篇综述详细介绍了我们目前的理解，通过脂质过氧化反应导致蛋白质羰基化的反应性醛的产生，重点是与4-羟基壬烯醛蛋白质修饰相关的病理生理因素。此外，概述了用于研究蛋白质羰基化的生理影响的体外和体内模型系统。最后，更新的方法通常用于表征蛋白质修饰的活性醛提供了一个概述的分离技术，质谱和计算生物学。很明显，在这一领域的研究采用最先进的蛋白质组学，质谱和计算生物学正在迅速发展，并产生有关蛋白质羰基化的分子机制及其与氧化应激相关的疾病谱的关系的基础知识。
The sustained overproduction of reactive oxygen and nitrogen species results in an imbalance of cellular prooxidant-antioxidant systems and is implicated in numerous disease states, including alcoholic liver disease, cancer, neurological disorders, inflammation and cardiovascular disease. The accumulation of reactive aldehydes resulting from sustained oxidative stress and lipid peroxidation is an underlying factor in the development of these pathologies. Determining the biochemical factors that elicit cellular responses resulting from protein carbonylation remains a key element to developing therapeutic approaches and ameliorating disease pathologies. This review details our current understanding of the generation of reactive aldehydes via lipid peroxidation resulting in protein carbonylation, focusing on pathophysiologic factors associated with 4-hydroxynonenal-protein modification. Additionally, an overview of in vitro and in vivo model systems used to study the physiologic impact of protein carbonylation is presented. Finally, an update of the methods commonly used in characterizing protein modification by reactive aldehydes provides an overview of isolation techniques, mass spectrometry and computational biology. It is apparent that research in this area employing state-of-the-art proteomics, mass spectrometry and computational biology is rapidly evolving and yielding foundational knowledge concerning the molecular mechanisms of protein carbonylation and its relation to a spectrum of diseases associated with oxidative stress.
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影响因子：
4.1
作者：
Cai, Jian;Bhatnagar, Aruni;Pierce, William M., Jr.
通讯作者：
Pierce, William M., Jr.
DOI：
10.1074/mcp.m800070-mcp200
发表时间：
2009-04
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Codreanu SG;Zhang B;Sobecki SM;Billheimer DD;Liebler DC
通讯作者：
Liebler DC
影响因子：
3.3
作者：
Aksenov, MY;Aksenova, MV;Markesbery, WR
通讯作者：
Markesbery, WR
影响因子：
5.1
作者：
Burcham, Philip C.;Raso, Albert;Thompson, Colin A.
通讯作者：
Thompson, Colin A.
影响因子：
2.3
作者：
Aldini, Giancarlo;Gamberoni, Luca;Carini, Marina
通讯作者：
Carini, Marina