α-Glucosidase inhibitors from the bulb of Eleutherine americana
α-Glucosidase inhibitors from the bulb of Eleutherine americana
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DOI：
10.1016/j.foodchem.2011.03.021
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发表时间：
2011-09-15
期刊：
影响因子：
8.8
通讯作者：
Kawabata, Jun
中科院分区：
文献类型：
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作者：
Ieyama, Tomohiro;Gunawan-Puteri, Maria D. P. T.;Kawabata, Jun
治疗糖尿病的一种有效方法是通过利用α-葡糖苷酶抑制剂（AGI）来抑制碳水化合物消化。通过体外酶法测定预期草药，结果发现美洲Eleuplusamericana，其显示出有效的抑制活性。采用50%甲醇水溶液提取法，对E. americana进行层析，并进一步用制备高效液相色谱（HPLC）纯化活性组分，以分离抗α-葡萄糖苷酶的活性化合物。通过质谱和核磁共振分析确定了这些化合物的结构，并与文献数据进行了比较，结果表明这些化合物分别为刺五加醇（1）、刺五加苷A（2）和刺五加苷B（3）。综合各萘类化合物在总提取物中的含量和抑菌活性，E.对α-葡糖苷酶的抑制活性可能是化合物2的结果（IC 50 = 0.5 mM，产率= 5 mg/50 g植物样品，具有在其他AGI中从未发现的特征结构。AGIs在糖尿病的治疗中发挥着重要作用，因此这些结果可能为糖尿病治疗管理提供新的替代方案。(C)2011爱思唯尔有限公司保留所有权利。
One effective way to treat diabetes is by suppressing carbohydrate digestion due to the utilisation of alpha-glucosidase inhibitors (AGIs). The determination of prospective herbs, done in vitro by using enzyme assay, resulted in the finding of Eleutherine americana, which showed a potent inhibitory activity. A 50% aqueous methanol-soluble extract of bulbs of E. americana was chromatographed successively and the active fractions were further purified with preparative high performance liquid chromatography (HPLC) to isolate active compounds against alpha-glucosidase. Structure determination by mass and NMR analysis revealed that these isolated compounds were eleutherol (1), eleutherinoside A (2), and eleuthoside B (3) based on comparisons with the reference data. Considering the amount and the inhibitory activity of each naphthalene in the whole extracts, the bulb of E. americana inhibitory activity against alpha-glucosidase might be a result of compound 2 (IC50 = 0.5 mM, yield = 5 mg/50 g plant sample, with a characteristic structure which has never been found in other AGIs. AGIs play an important role for the treatment of diabetes, therefore these results may suggest novel alternatives for diabetes treatment management. (C) 2011 Elsevier Ltd. All rights reserved.