Immunological parameters in prophylactic sublingual immunotherapy in asymptomatic subjects sensitized to Japanese cedar pollen
Immunological parameters in prophylactic sublingual immunotherapy in asymptomatic subjects sensitized to Japanese cedar pollen
复制标题
对日本柳杉花粉敏感的无症状受试者预防性舌下免疫治疗的免疫学参数
DOI：
10.1016/j.alit.2014.07.001
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发表时间：
2015
影响因子：
6.8
通讯作者：
Takeuchi K.
中科院分区：
文献类型：
--
作者：
Yamanaka K;Shah SA;Sakaida H;Yamagiwa A;Masuda S;Mizutani H;Takeuchi K.
背景资料：本研究的目的是检查免疫学参数，重点IL-10的生产力，在预防性舌下免疫治疗（SLIT）在无症状受试者致敏日本雪松花粉（JCP）。方法：本研究作为一个随机，双盲，安慰剂对照，多中心试验的一部分，并进行了两个连续的花粉季节在2012年和2013年。目前的结果仅基于我们的机构。我们招募了29名在JCP散布时具有2级和更高水平的抗JCP特异性IgE且没有花粉症症状史的参与者。SLIT组在花粉季节接受标准化JCP提取物5个月。在花粉季节观察和判断症状的发展。通过流式细胞术分析产生IL-10的CD 4 + T（Trl）细胞、B细胞和单核细胞的百分比。JCP特异性IgE和总IgE也measured.Results：与安慰剂组相比，SLIT组2013年雪松花粉症的发病率显著降低。2012年，SLIT组中循环Tr 1细胞和产生IL-10的单核细胞的百分比显著增加。在2013年，SLIT组中循环Tr 1细胞和产生IL-10的B细胞的百分比显著增加。循环中产生IL-10的单核细胞的百分比显着降低安慰剂group.Conclusions：预防性SLIT是有效的预防花粉症的发展。诱导产生IL-10的T细胞、B细胞和单核细胞是SLIT预防无症状但致敏受试者的花粉症的重要机制。
Background: This study aims to examine the immunological parameters, focusing IL-10 productivity, in prophylactic sublingual immunotherapy (SLIT) in asymptomatic subjects sensitized to Japanese cedar pollen (JCP).Methods: This study was conducted as part of a randomized, double-blind, placebo-controlled, multiple center trial, and was performed for two consecutive pollen seasons in 2012 and 2013. The present results were based only on our institution. We recruited 29 participants with specific IgE against JCP of at class 2 and higher levels without history of the pollinosis symptoms at the time of JCP scattering. The SLIT group received standardized JCP extract for five months over the pollen season. We observed and judged development of the symptoms in the pollen season. The percentage of IL-10 producing CD4+ T (Trl) cells, B cells and monocytes were analyzed by flow cytometry. JCP specific IgE and total IgE were also measured.Results: The ratio of development of cedar pollinosis was significantly lower in the SLIT group compared to the placebo group in 2013. In 2012, the percentage of circulating Tr1 cells and IL-10 producing monocytes significantly increased in the SLIT group. In 2013, the percentage of circulating Tr1 cells and IL-10 producing B cells increased significantly in the SLIT group. The percentage of circulating IL-10 producing monocytes significantly decreased in the placebo group.Conclusions: Prophylactic SLIT is effective for prevention of the development of pollinosis. Induction of IL-10 producing T cells, B cells and monocytes is an important mechanism of SLIT for prevention of pollinosis in asymptomatic but sensitized subjects.