Simulation of hormone effects by polycations.
Simulation of hormone effects by polycations.
复制标题
模拟聚阳离子的激素效应。
作者：
J. Wolff;G. H. Cook
y - 1肾上腺肿瘤和1-10间质瘤细胞的甾体生成对大分子量范围的聚赖氨酸敏感。在低浓度的聚合物中，两种细胞系的类固醇生成都有5 - 10倍的刺激。这在较高的聚赖氨酸浓度下被消除。当聚合度为> 9-18时，单体浓度为~ 1 × 10−4M时，无论分子量如何，都能产生半最大刺激。在这些条件下，来自y - 1和1-10细胞的部分纯化膜中的腺苷酸环化酶被作为部分激动剂的聚赖氨酸刺激（与甾体生成相反，其反应可与ACTH1-24或腺苷获得的最大效果相媲美）。当以单体浓度表示时，所有分子量的聚赖氨酸的半最大刺激出现在5 × 10−4M。在类固醇形成的情况下，核糖核酸酶A是一种有效的刺激物，但精胺只刺激膜，而不是完整的细胞。在浓度……
Steroidogenesis in Y-l adrenal tumor and 1–10 Leydig tumor cells is sensitive to polylysines of a wide molecular weight range. At low concentrations of polymer there is a 5–10-fold stimulation of steroidogenesis in both cell lines. This is abolished at higher polylysine concentrations. With degrees of polymerization >9–18, half-maximal stimulation occurs at ∼1 × 10−4M monomer concentration irrespective of molecular weight. Adenylate cyclase in partially purified membranes from Y-l and 1–10 cells is stimulated by polylysines which act as partial agonists under these conditions (in contrast to steroidogenesis where responses were comparable to maximal effects obtained with ACTH1–24 or adenosine, respectively). Half-maximal stimulation for all molecular weights of polylysine occurred at 5 × 10−4M when the level is expressed as the concentration of monomer. As in the case of steroidogenesis, ribonuclease A is an effective stimulator but spermine stimulates only membranes and not intact cells. At concentration...