Characterization of Arginine Kinase in the Barnacle Amphibalanus Amphitrite and Its Role in the Larval Settlement
Characterization of Arginine Kinase in the Barnacle Amphibalanus Amphitrite and Its Role in the Larval Settlement
复制标题
藤壶 Amphitrite 中精氨酸激酶的特征及其在幼虫定居中的作用
DOI：
10.1002/jez.b.22678
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Pei-Yuan Qian
中科院分区：
文献类型：
--
作者：
Gen Zhang;Guo-Yong Yan;Xiao-Xue Yang;Yue-Him Wong;Jin Sun;Yu Zhang;Li-Sheng He;Ying Xu;Pei-Yuan Qian
能量代谢是藤壶幼虫定居的关键过程，但潜在的分子机制仍然不明确。精氨酸激酶（AK）主要参与无脊椎动物的能量代谢。到目前为止，它在藤壶中的作用尚未被研究。在本研究中，我们从 Amphibalanus amphitrite Darwin 中提取了一种针对 AK 的抗体，以表征 AK 在幼虫定居过程中的作用。在发育阶段中，AK在cypris阶段高表达。随着鲤鱼的衰老过程，AK水平下降。免疫染色结果显示，AK定位于鲤鱼的肌肉组织，包括触角、触角肌和胸肢。高浓度 AK 抑制剂（芦丁和槲皮素）处理后，幼虫沉降率降低，幼虫运动受到抑制。这些结果表明AK参与了A幼虫的沉降。安菲特里特通过介导肌肉组织的能量供应。此外，进一步分析表明p38 MAPK和NO/cGMP途径均正介导鲤鱼中AK的表达。
Energy metabolism is a key process in larval settlement of barnacles, but the underlying molecular mechanisms remain ambiguous. Arginine kinase (AK) mainly participates in energy metabolism in invertebrates. So far, its roles in barnacles have not been studied. In the present study, we raised an antibody against AK fromAmphibalanus amphitriteDarwin to characterize the roles of AK in the larval settlement process. Among the developmental stages, AK was highly expressed during the cypris stage. Along with the aging process in cyprids, the level of AK decreased. The immunostaining results showed that AK was localized to muscular tissues in cyprids, including antennules, antennular muscles, and thoracic limbs. The larval settlement rate decreased and larval movement was inhibited in response to treatments with high concentrations of AK inhibitors (rutin and quercetin). These results demonstrated that AK was involved in the larval settlement ofA. amphitritethrough mediating energy supply in muscle tissues. Moreover, further analysis indicated that both the p38 MAPK and NO/cGMP pathways positively mediated the expression of AK in cyprids.