Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata.
Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata.
复制标题
胶原酶金加 BP 蛋白酶在从人胰腺中分离胰岛中的评价。
DOI：
10.1080/19382014.2017.1417716
复制
发表时间：
2018
期刊：
影响因子：
2.2
通讯作者：
Al-Abdullah,IsmailH
中科院分区：
文献类型：
--
作者：
Khiatah,Bashar;Tucker,Amber;Chen,Kuan-Tsen;Perez,Rachel;Bilbao,Shiela;Valiente,Luis;Medrano,Leonard;Rawson,Jeffrey;Forouhar,Elena;Omori,Keiko;Kandeel,Fouad;Qi,Meirigeng;Al-Abdullah,IsmailH
选择最佳胰腺消化的酶是成功分离人胰岛的关键。本研究的目的是通过将其与两种市售酶Liberase MTF C/T（Roche）（n = 48）和胶原酶NB 1/NP（Serva）（n = 15）进行比较，评价使用胶原酶Gold + BP蛋白酶（VitaCyte）（n = 8）的疗效和结局。通过胰岛计数、存活率、葡萄糖刺激的耗氧率（OCR）和移植成功率评估分离结果。胰腺供体特征在测试的酶组之间在其BMI、胰腺重量、冷缺血时间（CIT）和HbA 1c方面没有显著差异。结果表明，VitaCyte酶（34.25 ± 5.4 mL）和Roche酶（55.25 ± 3.42 mL，p = 0.073）之间的消化组织体积无统计学显著性，但Serva酶（64.07 ± 7.95 mL，p = 0.020）具有显著性。有趣的是，胰岛产量在所有酶组之间没有统计学差异。此外，当将胰岛移植到NOD scid小鼠中时，VitaCyte酶组的糖尿病逆转率与所有酶组相似。总之，胶原酶Gold + BP蛋白酶的有效性与MTF C/T和胶原酶NB 1/NP酶相当;低成本可促进使用更多胰腺进行胰岛分离。
Selection of enzymes for optimal pancreas digestion is essential for successful human islet isolations. The aim of this study was to evaluate the efficacy and outcome of using Collagenase Gold plus BP protease (VitaCyte) (n = 8) by comparing it to two commercially available enzymes, Liberase MTF C/T (Roche) (n = 48) and Collagenase NB1/NP (Serva) (n = 15). The isolation outcomes were assessed by islet counting, viability, glucose-stimulated oxygen consumption rate (OCR), and successful graft-rate following transplantation in diabetic NOD scid mice. The pancreas donor characteristics were not significantly different between the tested enzyme groups regarding their BMI, pancreas weight, cold ischemia time (CIT) and HbA1c. The results show that digested tissue volume was not statistically significant between the VitaCyte enzyme (34.25 ± 5.4 mL) and the Roche enzyme (55.25 ± 3.42 mL, p = 0.073), however, this was significant with Serva enzyme (64.07 ± 7.95 mL, p = 0.020). Interestingly, the islet yields were not statistically different between all enzyme groups. Moreover, when islets were transplanted into NOD scid mice, the reversal rate of diabetes for the VitaCyte enzyme group was similar to all enzyme groups. In conclusion, the effectiveness of Collagenase Gold plus BP protease is comparable to the MTF C/T and the Collagenase NB1/NP enzymes; the low cost could facilitate the use of more pancreata for islet isolations.