Ethanol productivity of cryophilic basidiomycetous yeast Mrakia spp. correlates with ethanol tolerance
Ethanol productivity of cryophilic basidiomycetous yeast Mrakia spp. correlates with ethanol tolerance
复制标题
低温担子菌酵母 Mrakia spp 的乙醇生产率。
DOI：
10.1016/j.myc.2015.08.002
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发表时间：
2016
期刊：
影响因子：
1.4
通讯作者：
Hoshino T
中科院分区：
文献类型：
--
作者：
Tsuji M;Kudoh S;Hoshino T
27株担子菌酵母Mrakia spp.的湖泊沉积物和土壤周围的湖泊在Skarvsnes地区，东南极洲。根据核糖体内转录间隔区和D1/D2结构域的序列相似性，将分离菌株分为3个种：Mrakia blollopis（18株）、M. gelida（7株）和M.罗氏线虫（2株）。所有菌株在10 ℃下从葡萄糖产生乙醇。从27株分离物中选出6株，然后在100 g/l葡萄糖下测试乙醇发酵和乙醇耐受性。Mrakia blollopis SK-4和M. Blollopis NRI-4分别在发酵13 d和6 d内完全消耗葡萄糖，表现出较高的乙醇产量和乙醇耐受性。相比之下，M。blollopis AGK-3和M. Blollopis TKG 1 -2乙醇产量低，耐乙醇性差。这些结果表明不同Mrakia物种的乙醇产量可能取决于个体菌株的乙醇耐受性。© 2015日本真菌学会。出版社：Elsevier BV版权所有。
Twenty-seven strains of basidiomycetous yeast Mrakia spp. were collected from lake sediment and soil surrounding the lakes in the Skarvsnes area, East Antarctica. Based on sequence similarity of their ribosomal internal transcribed spacer regions and D1/D2 domains, the isolated strains were classified into three species; Mrakia blollopis (18 strains), M. gelida (7 strains), and M. robertii (2 strains). All of the strains produced ethanol from glucose at 10 C. Six strains selected from the 27 isolates were then tested for ethanol fermentation and ethanol tolerance at 100 g/l glucose. Mrakia blollopis SK-4 and M. blollopis NRI-4 completely consumed the glucose within 13 d and 6 d of fermentation respectively, and demonstrated high ethanol yields and ethanol tolerance. In contrast, M. blollopis AGK-3 and M. blollopis TKG1-2 exhibited low ethanol yields and poor ethanol tolerance. These results indicate that ethanol yields of differentMrakia species may depend on ethanol tolerance in individual strains.© 2015 The Mycological Society of Japan. Published by Elsevier BV All rights reserved.
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影响因子：
1.5
作者：
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DOI：
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DOI：
10.1271/bbb.130497
发表时间：
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期刊：
Bioscience, Biotechnology, and Biochemistry
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.7
作者：
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通讯作者：
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