Effect of miR-124 on neuronal apoptosis in rats with cerebral infarction through Wnt/β-catenin signaling pathway
Effect of miR-124 on neuronal apoptosis in rats with cerebral infarction through Wnt/β-catenin signaling pathway
复制标题
DOI：
10.26355/eurrev_201908_18556
复制
发表时间：
2019-08-01
影响因子：
3.3
通讯作者：
Qian, X-J
中科院分区：
文献类型：
--
作者：
Che, Q-Q;Huang, T.;Qian, X-J
目的：本研究旨在观察微核糖核酸（microribonucleic acid，miR）-124对脑梗死（cerebral infarction，CI）大鼠神经细胞凋亡的影响，进一步探讨miR-124在CI发生发展中的作用机制。采用随机数字表将60只成年雄性Wistar大鼠随机分为Sham组、CI组和CI + miR-124 mimics组。采用线栓法建立局灶性Cl模型。在成功建模后，将miR-124模拟物立体定向注射到大鼠的侧脑室中。术后24 h采用改良神经功能缺损评分（mNSS）对各组大鼠进行神经功能评分。同时采用氯化三苯基四氮唑（TTC）法测定脑组织梗死面积。B细胞淋巴瘤相关基因BcI-2、BcI-2相关X蛋白（Bax）的蛋白表达水平。Western blotting检测C-Caspase和T-Caspase的表达。免疫荧光染色检测caspase-3在脑组织中的表达和定位。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）检测各组细胞凋亡水平。结果：聚合酶链反应（PCR）结果显示，与Sham组相比，CI组miR-124的表达水平显著降低（P
OBJECTIVE: The aim of this study was to observe the influence of micro ribonucleic acid (miR)-124 on neuronal apoptosis in rats with cerebral infarction (Cl), and to further investigate the underlying mechanism of miR-124 in CI occurrence and development.MATERIALS AND METHODS: A total of 60 adult male Wistar rats were randomly divided into the Sham group, the CI group and the CI + miR-124 mimics group using a random number table. The focal Cl model was established using the suture-occluded method. After successful modeling, miR-124 mimics were stereotactically injected into the lateral ventricle of rats. 24 h after operation, the neurological function of rats in each group was scored using modified neurological severity score (mNSS). Meanwhile, the infarction area of brain tissues was evaluated by the triphenyl tetrazolium chloride (TTC) method. The protein expression levels of apoptosis-related genes, including B-cell lymphoma-2 (BcI-2), BcI-2 associated X protein (Bax). C-Caspase and T-Caspase, were detected via Western blotting. The expression and location of caspase-3 in brain tissues were detected via immunofluorescence staining. Moreover, the level of apoptosis in each group was detected via terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. In addition, the expression levels of the Wnt/beta-catenin signaling pathway-related proteins were detected via Western blotting.RESULTS: Polymerase Chain Reaction (PCR) results revealed that the expression level of miR-124 in the CI group was significantly decreased when compared with that of the Sham group (p