Genetic and Biochemical Characterization of Indole Biodegradation in Acinetobacter sp. Strain O153
Genetic and Biochemical Characterization of Indole Biodegradation in Acinetobacter sp. Strain O153
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DOI：
10.1128/aem.01453-17
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发表时间：
2017
影响因子：
4.4
通讯作者：
Mikas Sadauskas;Justas Vaitekūnas;Renata Gasparavičiūtė;R. Meškys
中科院分区：
文献类型：
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作者：
Mikas Sadauskas;Justas Vaitekūnas;Renata Gasparavičiūtė;R. Meškys
吲哚是一种具有重要生物化学意义的分子，既是物种间的信号分子，又是生物元素的组成部分。细菌吲哚降解已被证明为一些情况下，然而，很少有人知道有关基因和蛋白质参与这一过程。本研究报告的克隆和初步功能特性的基因（iif和蚂蚁簇），负责吲哚生物降解不动杆菌属菌株O 153。在体外用重组蛋白重建分解代谢级联，并为每种蛋白分配酶功能。降解始于氧化，由IifC和IifD黄素依赖性双组分加氧酶系统介导。靛蓝的形成被IifB阻止，最终产物-邻氨基苯甲酸-由IifA形成，IifA是一种在结构和功能上都与辅因子非依赖性加氧酶相当的酶。此外，Iif簇在广泛的细菌基因组中被鉴定，表明Iif介导的吲哚降解的潜在广泛性。本研究为微生物降解吲哚的遗传背景提供了新的视角。重要性这项研究的关键发现是鉴定基因，负责微生物降解吲哚，一种有毒的N -杂环化合物。大量的吲哚存在于城市废水和污水污泥中，从而提高了对消除这种污染物的有效和生态友好的方法的需求。将吲哚氧化成靛蓝的常见策略具有产生不溶性材料的主要缺点。本文报道了不动杆菌O 153（DSM 103907）的基因和蛋白质，为有效和无靛蓝的吲哚去除铺平了道路。此外，这项工作提出了可能的新手段吲哚介导的细菌相互作用，并为未来的吲哚代谢的研究提供了基础。
Indole is a molecule of considerable biochemical significance, acting as both an interspecies signal molecule and a building block of biological elements. Bacterial indole degradation has been demonstrated for a number of cases; however, very little is known about genes and proteins involved in this process. This study reports the cloning and initial functional characterization of genes ( iif and ant cluster), responsible for indole biodegradation in Acinetobacter sp. strain O153. Catabolic cascade was reconstituted in vitro with recombinant proteins and each protein was assigned an enzymatic function. Degradation starts with oxidation, mediated by IifC and IifD flavin-dependent two component oxygenase system. Formation of indigo is prevented by IifB and the final product – anthranilic acid – is formed by IifA, an enzyme which is both structurally and functionally comparable to cofactor-independent oxygenases. Moreover, the iif cluster was identified in the genomes of wide range of bacteria, suggesting the potential widespread of Iif-mediated indole degradation. This work provides novel insights into genetic background of microbial indole biodegradation. IMPORTANCE The key finding of this research is identification of genes, responsible for microbial biodegradation of indole, a toxic N -heterocyclic compound. A large amount of indole is present in urban waste water and sewage sludge raising a demand for efficient and eco-friendly means to eliminate this pollutant. A common strategy of oxidizing indole to indigo has a major drawback of producing insoluble material. Genes and proteins of Acinetobacter sp. strain O153 (DSM 103907) reported here pave the way for effective and indigo-free indole removal. In addition, this work suggests possible novel means of indole-mediated bacterial interactions and provides the basis for future research on indole metabolism.