The Effects of Goldblatt Hypertension on Development of the Glomerular Lesions of Diabetes Mellitus in the Rat
The Effects of Goldblatt Hypertension on Development of the Glomerular Lesions of Diabetes Mellitus in the Rat
复制标题
戈德布拉特高血压对大鼠糖尿病肾小球病变发展的影响
作者：
S. Mauer;M. Steffes;S. Azar;Susan Kupcho Sandberg;David M. Brown
经典Goldblatt（两肾）高血压大鼠由链脲佐菌素诱导糖尿病。糖尿病4个月后，高血压糖尿病大鼠的未夹肾肾小球有显着增加糖尿病的变化，包括系膜基质增厚和系膜免疫球蛋白（IgG和IgM）和补体（C3）的定位，当与对侧夹肾肾小球相比。此外，高血压糖尿病动物的未夹肾的肾小球比正常血压糖尿病大鼠的肾小球有更多的系膜增厚和IgG和IgM染色。尽管高血压糖尿病大鼠的肾小球系膜增厚比正常血压糖尿病大鼠的肾小球少，但这并没有达到统计学意义。然而，与血压正常的糖尿病动物的肾小球相比，这些肾小球确实具有显著较少的IgG、IgM和C3染色。夹闭和未夹闭的非糖尿病高血压大鼠肾小球系膜厚度与正常动物无差异。然而，有较少的系膜IgG染色在夹比在非糖尿病高血压大鼠的肾脏或正常动物的肾脏未夹。我们解释这些结果意味着肾单位血流动力学的改变与糖尿病状态联合收割机影响大鼠糖尿病肾小球病变的发展速度。
Rats with classic Goldblatt (two-kidney) hypertension had diabetes induced by streptozotocin. After four months of diabetes, glomeruli of the unclipped kidney of hypertensive diabetic rats had markedly increased diabetic changes, including mesangial matrix thickening and mesangial immunoglobulin (IgG and IgM) and complement (C3) localization, when compared with glomeruli of the contralateral-clipped kidneys. Further, glomeruli of the unclipped kidneys of hypertensive diabetic animals had more mesangial thickening and IgG and IgM staining than glomeruli of normotensive diabetic rats. Although glomeruli of clipped kidneys in hypertensive diabetic rats had less mesangial thickening than glomeruli of normotensive diabetic rats, this did not reach statistical significance. However, these glomeruli did have significantly less IgG, IgM, and C3 staining compared with glomeruli of normotensive diabetic animals. Mesangial thickness in glomeruli of clipped and unclipped nondiabetic hypertensive rats did not differ from that in normal animals. However, there was less mesangial IgG staining in clipped than in unclipped kidneys of nondiabetic hypertensive rats or in kidneys of normal animals. We have interpreted these results to imply that alterations in nephron hemodynamics combine with the diabetic state to influence the rate of development of diabetic glomerulopathy in rats.