Time-dependent effect of E-coli LPS in spleen DC activation in vivo: Alteration of numbers, expression of co-stimulatory molecules, production of pro-inflammatory cytokines, and presentation of antigens
Time-dependent effect of E-coli LPS in spleen DC activation in vivo: Alteration of numbers, expression of co-stimulatory molecules, production of pro-inflammatory cytokines, and presentation of antigens
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大肠杆菌 LPS 在体内脾 DC 激活中的时间依赖性效应：数量的改变、共刺激分子的表达、促炎细胞因子的产生和抗原的呈递
DOI：
10.1016/j.molimm.2017.02.017
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发表时间：
2017-05-01
影响因子：
3.6
通讯作者：
Jin, Jun-O.
中科院分区：
文献类型：
--
作者：
Xu, Li;Kwak, Minseok;Jin, Jun-O.
脂多糖（LPS）是一种众所周知的树突状细胞（DC）刺激物。然而，在体内脾DC成熟的大肠杆菌（大肠杆菌）LPS尚未得到充分的研究。在本研究中，我们研究了LPS对小鼠脾脏DCs及其亚群的激活的影响，并以时间依赖的方式在体内进行。LPS处理后6 h和12 h，脾脏常规DC（cDC）的频率、数量和迁移率均增加。脾脏中DC数量的增加随着DC的凋亡而逐渐减少。LPS处理后18 h，脾脏cDCs及其亚群共刺激分子表达水平最高，LPS处理后3 h，脾脏cDCs及其亚群细胞内促炎细胞因子表达水平最高。LPS处理后3 ~ 12 h，脾脏cDCs及其亚群的抗原提呈水平逐渐升高，但18 h后迅速下降。因此，通过强调时间在小鼠体内LPS刺激脾脏DC中的重要性，我们的数据提供了一个模型，当考虑在实验和临床应用中操纵DC功能时，免疫学家可以使用该模型。(C)2017由Elsevier Ltd.出版
Lipopolysaccharide (LPS) is a well-known stimuli of dendritic cells (DCs). However, in vivo spleen DC maturation by Escherichia coli (E.coli) LPS has not been fully investigated. In this study, we examined the effect of LPS on the activation of spleen DCs and its subsets in a time-dependent manner on mice in vivo. The frequency, number and migration of spleen conventional DCs (cDCs) were increased 6 and 12 h after completion of LPS treatment. Those increased DC numbers in spleen were then gradually decreased with apoptosis of the DCs. The highest levels of co-stimulatory molecule expression in the spleen cDCs and their subsets occurred 18 h after LPS treatment, while the pro-inflammatory cytokines reached their maximum in the intracellular levels of the spleen cDCs and their subsets 3 h after LPS treatment. The antigen presentation of the spleen cDCs and their subsets increased gradually from 3 to 12 h after LPS treatment, but those levels decreased rapidly after 18 h post-LPS treatment. Thus, by highlighting the importance of time in the stimulation of spleen DCs by LPS in mice in vivo, our data provided a model that could be used by immunologists when considering the manipulation of DC functions in vivo for experimental and clinical applications. (C) 2017 Published by Elsevier Ltd.