The effect of antisera to bovine LH in hysterectomized and intact heifers.
The effect of antisera to bovine LH in hysterectomized and intact heifers.
复制标题
抗血清对子宫切除和完整小母牛 LH 的影响。
作者：
R. Snook;M. Brunner;R. Saatman;W. Hansel
将抗牛LH的抗血清注射到切除卵巢的小母牛中，可引起黄体重量、总孕酮、总孕激素和20 β-羟基孕酮4-烯-3-酮（204-01）浓度的显著降低，但对孕酮浓度没有影响。垂体前叶的促性腺激素分泌细胞明显脱颗粒，垂体LH含量降低。甲状腺上皮细胞高度降低，表明抗血清也含有抗TSH。周期的第2天至第6天的周期小母牛的抗牛LH的管理引起黄体重量和总孕酮含量的显着下降，但孕酮浓度只有轻微的下降。在切除睾丸的抗LH给药小母牛的黄体中，退化的组织学体征明显，但在对照和给药周期动物的黄体之间几乎没有差异。从周期的第2天至第6天每天注射50 ml抗牛LH，增加了第11天去除黄体和恢复发情之间的间隔。从周期的第12天至第16天和第16天至第20天每天注射50 ml抗牛LH，分别将治疗周期的长度增加至28.5天和36.7天。两组治疗后第一个周期均明显缩短。周期长度的增加主要是由于卵泡未能发育至成熟大小，而不是由于黄体寿命的延长。这些实验的结果表明，LH是卵泡发育和成熟，诱导发情，以及维持黄体所必需的。
Injection of antiserum to bovine LH into hysterectomized heifers caused significant decreases in corpus luteum weight, total progesterone, total progestins and 20�-hydroxypregn4-en-3-one (204-01) concentration but had no effect on progesterone concentration. Gonadotropin secreting cells in the anterior pituitaries of these heifers were markedly degranulated and pituitary LH contents lowered. Thyroid epithelial cell heights were lowered suggesting that the antiserum also contained anti-TSH. The administration of antibovine LH to cyclic heifers from Day 2 to Day 6 of the cycle caused a significant decrease in corpus luteum weight and total progesterone content, but only a slight decrease in progesterone concentration. Histological signs of regression were clearly evident in the corpora lutea of the hysterectomized anti-LH treated heifers, but little difference could be noted between the corpora lutea of control and treated cyclic animals. Injection of 50 ml of antibovine LH daily from Day 2 to Day 6 of the cycle increased the interval between corpus luteum removal at Day 11 and return to estrus. Injections of 50 ml of antibovine LH daily from Day 12 to Day 16 and from Day 16 to Day 20 of the cycle increased the lengths of the treatment cycles to 28.5 and 36.7 days, respectively. The first post-treatment cycle in both groups was significantly shortened. The increase in cycle length was primarily due to a failure of follicles to develop to mature size, rather than to prolongation of the life span of the corpora lutea. The results of these experiments indicated that LH is required for follicular development and maturation and for the induction of estrus, as well as for corpus luteum maintenance.