A Quantitative Test for Biotin and Observations Regarding its Occurrence and Properties
A Quantitative Test for Biotin and Observations Regarding its Occurrence and Properties
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生物素的定量测试及其发生和性质的观察
DOI：
10.1021/ja01858a052
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发表时间：
1940
影响因子：
15
通讯作者：
R. Williams
中科院分区：
文献类型：
--
作者：
E. Snell;R. E. Eakin;R. Williams
在对生物酸1的研究过程中，我们发现维生素Be可以作为酵母的营养物2，并引导我们将纯维生素引入基础培养基。(与此同时，维生素Be对酵母菌的作用也在其他地方被独立发现。3)在这种改良的基础培养基中，酵母对少量肝制剂的补充物更加敏感。不久之后，在Kogl教授的好意下，我们得到了一个生物锡的样本，一些测试表明，在这种培养基中，酵母对生物素有特殊的反应。
In the course of studies on biotic acid1 we found that vitamin Be serves as a yeast nutrilite2 and were led to introduce the pure vitamin into our basal culture medium.(The effect of vitamin Be on yeast was independently discoveredelsewhere at the same time. 3) In this modified basal me-dium, yeast became much more sensitive to addenda of small amounts of liver preparations. Shortly after this, through the kindness of Profes-sor Kogl, we were furnished with a sample ofbio-tin, and a few tests demonstrated that in this medium the yeast was responding specifically to biotin.