Expression of Ezrin correlates with lung metastasis in Chinese patients with osteosarcoma.
Expression of Ezrin correlates with lung metastasis in Chinese patients with osteosarcoma.
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DOI：
10.25011/cim.v32i2.6036
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发表时间：
2009-04
期刊：
影响因子：
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通讯作者：
Shen Xu-dong;Shen Zan;Zheng Shui-er;Tang Li-na;Yuan Wen-xi;Lin Feng;Yao Yang
中科院分区：
文献类型：
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作者：
Shen Xu-dong;Shen Zan;Zheng Shui-er;Tang Li-na;Yuan Wen-xi;Lin Feng;Yao Yang
目的探讨Ezrin、CD44和Six1基因在中国人骨肉瘤组织中的表达及其与预后的关系。方法应用荧光定量实时定量PCR技术检测32例骨肉瘤患者组织、10例癌旁正常组织及MG 63骨肉瘤细胞系中Ezrin、CD44和Six1基因的mRNA水平。根据临床资料分析肺转移与总生存时间的关系。结果Ezrin和Six1基因在骨肉瘤组织中的mRNA水平明显高于癌旁正常组织（P= 0.015，0.025）。Ezrin、CD44和Six1基因mRNA水平与Enneking GTM临床分期密切相关，而与性别、年龄、部位和病理类型无关。Ezrin基因高表达与中国人骨肉瘤患者的无肺转移和总生存时间有关（P < 0.001）。结论Ezrin可能是骨肉瘤肺转移的预测因子，而CD44和Six1可能不是。需要进行进一步的大样本研究以确认Ezrin作为新的治疗靶点的潜在价值。
PURPOSE To determine the prognostic value of the expression of Ezrin, CD44 and Six1 genes in osteosarcoma tissues of Chinese patients. METHODS Fluorescent quantitative real-time PCR was applied to study the mRNA levels of Ezrin, CD44 and Six1 genes in 32 osteosarcoma patient samples and 10 adjacent normal tissues and MG63 osteosarcoma cell lines. The analysis of relationships between pulmonary metastasis and overall survival time were carried out based on the clinical data. RESULTS mRNA levels of Ezrin and Six1 genes in osteosarcoma tissues were higher than those in adjacent normal tissues (P=0.015, 0.025). The mRNA levels of Ezrin, CD44 and Six1 genes were closely correlated with Enneking GTM clinical staging, while no correlations were demonstrated between the mRNA level of these genes with sex, age, location or pathological types. In addition, we demonstrated that the high mRNA level of Ezrin gene was related to shorter lung metastasis-free and overall survival time of the Chinese patients with osteosarcoma (P < 0.001). CONCLUSION Our data suggest that Ezrin, but not CD44 and Six1, could be a prognostic factor and a predictor of potential lung metastasis in osteosarcoma. Further large sample studies need to be done to confirm the potential value of Ezrin as a new therapeutic target.