Evaluation of the robustness of the enzymatic hydrolysis in batch and continuous mode by a central composite design
Evaluation of the robustness of the enzymatic hydrolysis in batch and continuous mode by a central composite design
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DOI：
10.1111/jfpp.13330
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发表时间：
2018
影响因子：
2.5
通讯作者：
N. Abdi;O. Kitous;H. Grib;M. Drouiche;Hakim Lounici;N. Drouiche;N. Mameri
中科院分区：
文献类型：
--
作者：
N. Abdi;O. Kitous;H. Grib;M. Drouiche;Hakim Lounici;N. Drouiche;N. Mameri
这项工作的主要目的是研究酶促反应的能力。为了进行实验，需要控制变化，以最大限度地减少变化，从而保持最佳工作点。为了实现这一目标，中心复合设计考虑了所有可能有助于创建符合有效性标准的数学模型的相互作用效应。该模型显示比连续模式更适合间歇模式的糖化实验。在批量模式下，获得了接近于 1 的相关系数 (R2 = .98)。此外，根据验证标准，所得数学模型令人满意。对于连续模式，获得的系数值为R2 = .83，表明该模型研究酶促反应的能力比分批模式的分析条件要差。实际应用 研究了酶促反应在小的实验条件变化下保持其性能的能力。中心组合设计符合标准有效性。获得了接近于 1 的相关系数 (R2 = .98)。
The main purpose of this work is to study the ability of the enzymatic reaction. For the performance of the experiment, it is desirable to control variations so that they are minimized to the maximum extend and therefore an optimum operating point can be maintained. To achieve this target, the central composite design takes into account all interaction effects that may be useful for creating a mathematical model in agreement with the validity criteria. The model showed to be more suitable for the saccharification experiments in batch mode than in continuous mode. In batch mode, a correlation coefficient close to unity (R2 = .98) was obtained. Moreover, the resulting mathematical model was satisfactory in accordance with the validation criteria. For continuous mode, the coefficient value obtained was R2 = .83 indicating that the model to study the ability of the enzymatic reaction was inferior than the batch mode conditions of the analysis. Practical applications The ability of the enzymatic reaction to maintain its performance at small experimental condition variations was studied. The central composite design conformed to criterion validity. A correlation coefficient close to unity (R2 = .98) was obtained.