Expression of the p16 and Ki-67 in relation to the grade of cervical intraepithelial neoplasia and high-risk human papillomavirus infection.
Expression of the p16 and Ki-67 in relation to the grade of cervical intraepithelial neoplasia and high-risk human papillomavirus infection.
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DOI：
10.3802/jgo.2008.19.3.162
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发表时间：
2008-09-01
影响因子：
3.9
通讯作者：
Kim, Jong Wook
中科院分区：
文献类型：
--
作者：
Nam, Eun Ji;Kim, Jae Wook;Kim, Jong Wook
目的：探讨p16(INK4a)和Ki-67在宫颈上皮内瘤变(CIN)中的表达及其与高危人乳头状瘤病毒(HPV)感染的关系。CIN标本(CIN1，12例；CIN2，6例；CIN3，13例)均经阴道镜引导活检(CDB)或环状电切术(LEEP)获得。用免疫组织化学方法检测p16和Ki-67的表达。免疫组织化学染色结果分为0、1、2、3级。高危型HPV检测采用HPV基因分型或杂交捕获-II试验。
OBJECTIVE: The purposes of this study were to evaluate the expression of p16(INK4a) (referred as to p16) and Ki-67 in cervical intraepithelial neoplasia (CIN), and the correlation between high-risk human papillomavirus (HPV) infection and the above biomarkers.METHODS: We analyzed 31 patients who were diagnosed with CIN at Kwandong University Myongji Hospital from October 2006 to September 2007. CIN specimens (CIN1, 12; CIN2, 6; CIN3, 13) were obtained by colposcopy-directed biopsy (CDB) or loop electrical excision procedure (LEEP). The expressions of p16 and Ki-67 were evaluated by immunohistochemical methods with antibodies to p16 and Ki67. The immunohistochemical staining results were classified into four grades: 0, 1, 2 and 3. HPV genotyping or Hybrid Capture-II test was used to detect high-risk HPV.RESULTS: The expression of p16 (p