Expression of fibronectin-binding integrins in gingival epithelium in drug-induced gingival overgrowth.
Expression of fibronectin-binding integrins in gingival epithelium in drug-induced gingival overgrowth.
复制标题
药物诱导的牙龈过度生长中牙龈上皮中纤连蛋白结合整合素的表达。
DOI：
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复制
发表时间：
2007
影响因子：
3.5
通讯作者：
H. Larjava
中科院分区：
文献类型：
--
作者：
P. Walsh;L. Häkkinen;H. Pernu;M. Knuuttila;H. Larjava
背景与目的
牙龈增生是硝苯地平和环孢菌素药物的副作用。整合素是介导细胞粘附、调节细胞增殖并参与组织纤维化调节的跨膜糖蛋白。本研究的目的是调查上皮细胞整合素的表达是否与药物诱导的牙龈过度生长的发展有关。
材料和方法
使用了服用硝苯地平、环孢菌素或两种药物组合的患者的人类牙龈活检。α 5 β 1，α v β 1和α v β 6整合素，和纤连蛋白的细胞外结构域A的表达，使用免疫组化定位在冷冻切片。
结果
在联合用药组中，β 1、α 5 β 1和α v β 6整合素的活化构象在口腔上皮的不同位置更频繁地表达。细胞外结构域A的纤连蛋白，α 5 β 1和α v β 6整合素的配体，表达在所有组的结缔组织。在对照组、硝苯地平组和环孢霉素诱导的牙龈增生组的基底角质形成细胞周围也有表达，但在联合用药组中没有表达。没有发现炎症和整合素表达之间的关系。
结论
结果表明，在药物诱导的牙龈过度生长的上皮中，某些整合素的表达上调，它们可以参与控制延长的网脊和组织纤维化的形成。
BACKGROUND AND OBJECTIVE
Gingival overgrowth is a side-effect of nifedipine and cyclosporin medications. Integrins are transmembrane glycoproteins that mediate cell adhesion, regulate cell proliferation and participate in the regulation of tissue fibrosis. The aim of this study was to investigate whether expression of epithelial cell integrins is linked to the development of drug-induced gingival overgrowth.
MATERIAL AND METHODS
Human gingival biopsies of patients taking nifedipine, cyclosporin, or a combination of both medications, were used. Expression of the alpha5beta1, alphavbeta1 and alphavbeta6 integrins, and of cellular extra domain A of fibronectin, was localized in frozen sections using immunohistochemistry.
RESULTS
The activated conformation of the beta1, alpha5beta1 and alphavbeta6 integrins were more frequently expressed in distinct locations in the oral epithelium in the combined drug group. Cellular extra domain A of fibronectin, a ligand for both alpha5beta1 and alphavbeta6 integrins, was expressed within the connective tissue of all groups. It was also expressed around the basal keratinocytes of the control, nifedipine and cyclosporin-induced gingival overgrowth groups, but not in the combined medication group. No relationship between the presence of inflammation and integrin expression was found.
CONCLUSION
The results indicate that expression of certain integrins is up-regulated in the epithelium of drug-induced gingival overgrowth where they could participate in controlling the formation of elongated rete ridges and tissue fibrosis.
DOI：
10.1016/s0021-9258(18)68822-2
发表时间：
1988-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Roberts;T. Birkenmeier;J. McQuillan;S. K. Akiyama;S. Yamada;W. T. Chen;K. Yamada;J. McDonald
通讯作者：
C. Roberts;T. Birkenmeier;J. McQuillan;S. K. Akiyama;S. Yamada;W. T. Chen;K. Yamada;J. McDonald
影响因子：
15.9
作者：
Bata-Csorgo, Z;Cooper, KD;Hammerberg, C
通讯作者：
Hammerberg, C
药物引起的牙龈过度生长中的结缔组织生长因子。
影响因子：
4.3
作者：
Uzel,MI;Kantarci,A;Hong,HH;Uygur,C;Sheff,MC;Firatli,E;Trackman,PC
通讯作者：
Trackman,PC