Changes in emotional behavior produced by orexin microinjections in the paraventricular nucleus of the thalamus
Changes in emotional behavior produced by orexin microinjections in the paraventricular nucleus of the thalamus
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丘脑室旁核微量注射食欲素引起的情绪行为变化
DOI：
10.1016/j.pbb.2009.12.016
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发表时间：
2010-03-01
影响因子：
3.6
通讯作者：
Kirouac, Gilbert J.
中科院分区：
文献类型：
--
作者：
Li, Yonghui;Li, Sa;Kirouac, Gilbert J.
丘脑中线室旁核（PVT）支配杏仁核的延伸区域，已知杏仁核在情绪行为的表达中起关键作用。在这项研究中，食欲素（hypocretins），这对PVT中的神经元具有兴奋作用，在中线丘脑的微量注射被用来调查，如果PVT调制的情绪行为的表达在开放领域。首先，在将新物体放置在开放视野的中心之前和之后，在食欲素处理的大鼠中检查与新奇性相关的接近-回避倾向（访问中心区域的次数和持续时间）。其次，行为学行为（饲养，运动，冻结，梳理）在开放领域的表达被用来确定食欲素对情绪的影响。在PVT中微量注射食欲素-A（OXA）或食欲素-B（OXB）减少了对中心区域和新物体的探索，表明食欲素治疗的大鼠中中心区域和物体具有更多的厌恶性质。在PVT中的OXA和OXB微量注射增加了指示负面情绪状态的冻结和梳理行为的表达。结果表明，在PVT中微量注射食欲素使测试情境更令人厌恶，并产生回避行为。这表明食欲素可能作用于PVT，以调节与消极情绪状态相关的行为。(C)2010年爱思唯尔公司All rights reserved.
The paraventricular nucleus of the midline thalamus (PVT) innervates areas of the extended amygdala known to play a key role in the expression of emotional behaviors. In this study, microinjections of orexins (hypocretins), which have excitatory actions on neurons in the PVT, in the midline thalamus were used to investigate if the PVT modulates the expression of emotional behavior in the open field. First, the approach-avoidance tendency (number and duration of visit to the center area) associated with novelty was examined in orexin treated rats before and after placing a novel object in the center of the open field. Second, the expression of ethological behaviors (rearing, locomotion, freezing, and grooming) in the open field was used to determine the effects of orexins on emotionality. Microinjections of orexin-A (OXA) or orexin-B (OXB) in the PVT decreased exploration of the center area and the novel object indicating that the center area and the object had more aversive properties in orexin treated rats. Both OXA and OXB microinjections in the PVT increased the expression of freezing and grooming behaviors which are indicative of a negative emotional state. The results indicate that microinjections of orexins in the PVT made the test situation more aversive and produced avoidance behaviors. This suggests that orexins may act at the PVT to modulate behaviors associated with a negative emotional state. (C) 2010 Elsevier Inc. All rights reserved.