Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli
Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli
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大肠杆菌中参与包膜蛋白分泌的新基因（secA）和基因产物的鉴定
DOI：
10.1128/jb.150.2.686-691.1982
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发表时间：
1982
影响因子：
3.2
通讯作者：
J. Beckwith
中科院分区：
文献类型：
--
作者：
D. Oliver;J. Beckwith
我们构建了 lambda 特异性转导噬菌体，其携带参与某些包膜蛋白分泌的大肠杆菌基因 (secA)。这些噬菌体已被用来证明 secA 是 envA 顺时针一侧的新基因。先前描述的 secA 突变 secA5l(Ts) (D. B. Oliver 和 J. Beckwith, Cell 25:765-772, 1981)对于野生型等位基因是隐性的。我们还在转导噬菌体携带的基因中分离了 Tn5 插入，以进一步定义该基因。这些噬菌体用于感染紫外线照射的细胞，以鉴定 secA 基因产物为 92 千道尔顿的多肽，并显示 secA 的转录相对于大肠杆菌遗传图谱是顺时针方向。
We have constructed lambda specialized transducing phages which carry an Escherichia coli gene (secA) involved in the secretion of certain envelope proteins. These phage have been used to show that secA is a new gene to the clockwise side of envA. The secA mutation previously described, secA5l(Ts) (D. B. Oliver and J. Beckwith, Cell 25:765-772, 1981), is recessive to the wild-type allele. We have also isolated Tn5 insertions in the gene carried on the transducing phage to further define the gene. These phage were used to infect UV-irradiated cells to allow the identification of the secA gene product as a 92-kilodalton polypeptide and to show that transcription of secA is clockwise relative to the E. coli genetic map.