Expression of the human erythrocyte glucose transporter in transitional cell carcinoma of the bladder
Expression of the human erythrocyte glucose transporter in transitional cell carcinoma of the bladder
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DOI：
10.1016/s0090-4295(99)00474-4
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发表时间：
2000-03-01
期刊：
影响因子：
2.1
通讯作者：
Kim, YW
中科院分区：
文献类型：
--
作者：
Chang, SG;Lee, SJ;Kim, YW
目标。先前的研究表明，在肿瘤中，葡萄糖的摄取和使用比在正常细胞和组织中更为普遍。我们推测人红细胞葡萄糖转运蛋白（Glut-1）在膀胱移行细胞癌（TCC）中表达升高，其表达程度可能与恶性程度相关。对正常膀胱组织（5例）、良性乳头状瘤组织（10例）、浅表肿瘤组织（48例）、侵袭性肿瘤组织（31例）的Glut-1蛋白进行免疫染色。采用免疫反应性分级系统：肿瘤细胞无免疫反应性= 0；小于10%的肿瘤细胞免疫反应性= 1+；10% ~ 50%的肿瘤细胞免疫反应性= 2+；大于50%的肿瘤细胞免疫反应性= 3+。Glut-1蛋白的免疫染色在正常膀胱和良性乳头瘤样本中未表达，但在63.0%（73例中的46例）的TCC样本中表达。在Glut-1蛋白的表达模式中，浅表TCC呈局灶性染色，浸润性TCC在肿瘤巢中呈弥漫性染色。浸润性TCC中Glut-1蛋白的表达水平明显高于浅表性TCC (P = 0.002)，高核级TCC中Glut-1蛋白表达水平明显高于低核级TCC （P = 0.007）。在表面TCC样品中。膀胱肿瘤复发率与Glut-1蛋白表达无显著相关性（P = 0.40）。我们的研究结果表明，Glut-1蛋白在正常膀胱粘膜和良性病变中不表达，Glut-1蛋白的表达与膀胱TCC的肿瘤进展密切相关，而Glut-1蛋白的表达与浅表性膀胱TCC的复发率无关。(C) 2000, Elsevier Science Inc.；
Objectives. It has previously been shown that glucose uptake and use is more prevalent in carcinomas than in normal cei Is and tissues. We hypothesized that human erythrocyte glucose transporter (Glut-1) expression is increased in bladder transitional cell carcinoma (TCC) and that the grade of expression might correlate with the degree of malignancy.Methods. Immunostaining of Glut-1 protein was studied in normal bladder (5 cases), benign papilloma (10 cases), superficial tumor (48 cases), and invasive tumor (31 cases) tissue. The immunoreactivity grading system used was as follows: absence of immunoreactivity in tumor cell = 0; less than 10% of the tumor cells immunoreactive = 1+; 10% to 50% of the tumor cells immunoreactive = 2+; and greater than 50% of the tumor cells immunoreactive = 3+.Results. Immunostaining of Glut-1 protein was not expressed in the normal bladder or benign papilloma samples, but it was expressed in 63.0% (46 of 73) of the TCC samples. In the pattern of expression of Glut-1 protein, superficial TCC was stained focally, but invasive TCC was stained diffusely in the tumor nests. The grade of Glut-1 protein expression increased more significantly in the invasive TCC than in the superficial TCC samples (P = 0.002) and more significantly in the high nuclear grade than in the low nuclear grade samples (P = 0.007). In the superficial TCC samples. the bladder tumor recurrence rate did not significantly correlate with Glut-1 protein expression (P = 0.40).Conclusions. Our results suggest that the Glut-1 protein is not expressed in normal bladder mucosa and benign lesions, that Glut-1 protein expression is strongly associated with neoplastic progression in bladder TCC, and that Glut-1 expression does not correlate with the recurrence rate in superficial bladder TCC. (C) 2000, Elsevier Science Inc.