Activation of orexin signal in basal forebrain facilitates the emergence from sevoflurane anesthesia in rat
Activation of orexin signal in basal forebrain facilitates the emergence from sevoflurane anesthesia in rat
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基底前脑中食欲素信号的激活促进大鼠从七氟醚麻醉中苏醒
DOI：
10.1016/j.npep.2009.04.006
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发表时间：
2009-06-01
期刊：
影响因子：
2.9
通讯作者：
Xiong, Lize
中科院分区：
文献类型：
--
作者：
Dong, Hailong;Niu, Jiyuan;Xiong, Lize
食欲素能系统可能在麻醉-觉醒的调节中起重要作用。然而，哪些区域或哪些途径介导食欲素的作用仍不清楚。在本研究中，我们探讨了是否在基底前脑（BF）的食欲素信号激活可能会改变脑电图活动，诱导和苏醒时间七氟醚麻醉大鼠。在1.0最低肺泡浓度（2.4%）的七氟醚麻醉下，将食欲素A或食欲素B注入BF，同时测量脑电图（EEG）。在BF内分别注入食欲素受体激动剂（orexin-A或orexin-B）或拮抗剂（SB-334867 A）后，测定七氟醚麻醉诱导时间和苏醒时间。我们发现，在七氟醚麻醉下，给予orexin-A（30，100 pmol）和orexin-B（100 pmol）改变了大鼠觉醒EEG的爆发和抑制模式。与orexin-B相比，低剂量orexin-A诱发的脑电觉醒程度更高。基底动脉内微量注射食欲素-A缩短了苏醒时间，而基底动脉内微量注射SB-334867 A（5 μ g，20 μ g）延迟了七氟烷麻醉的苏醒时间，而不改变麻醉诱导。这些结果表明，在基底前脑，胆碱能腹侧上升唤醒系统的中间区域，食欲素信号在麻醉-唤醒调节中起着至关重要的作用。(C)2009爱思唯尔有限公司版权所有。
Orexinergic system may play an important role in the regulation of anesthesia-arousal. However, which region or which pathway mediated the effect of orexins was still unclear. In current study, we investigated whether activation of orexin signals in basal forebrain (BF) may alter electroencephalographic activity, induction and emergence time to sevolflurane anesthesia in rats. Either orexin-A or orexin-B was injected into the BF while measuring electroencephalogram (EEG) under 1.0 minimum alveolar concentration (2.4%) sevoflurane anesthesia. The induction and emergence time of sevoflurane anesthesia were measured respectively after an injection of orexin receptor agonist (orexin-A or orexin-B) or antagonist (SB-334867A) into the BF also. We found that the administration of orexin-A (30, 100 pmol) and orexin-B (100 pmol) changed the burst and suppression patterns to arousal EEG in rat under sevoflurane anesthesia. Comparing with orexin-B, injection of lower dose of orexin-A induced more arousal EEG. Intrabasalis microinjection of orexin-A shorted the emergence time, whereas intrabasalis microinjection of SB-334867A (5 mu g, 20 mu g) delayed the emergence time to sevoflurane anesthesia, without changing anesthetic induction. These findings indicate that the orexin signals in basal forebrain, a middle region of the cholinergic ventral ascending arousal system, plays a crucial role in the anesthesia-arousal regulation. (C) 2009 Elsevier Ltd. All rights reserved.