Developmentally regulated collagen/integrin interactions confer adhesive properties to early postnatal neural stem cells
Developmentally regulated collagen/integrin interactions confer adhesive properties to early postnatal neural stem cells
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DOI：
10.1016/j.bbagen.2014.01.021
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发表时间：
2014-08-01
影响因子：
3
通讯作者：
Forsberg-Nilsson, Karin
中科院分区：
文献类型：
--
作者：
Bergstrom, Tobias;Holmqvist, Karin;Forsberg-Nilsson, Karin
背景：越来越明显的是，细胞外基质在神经干生态位中起着重要的调节作用。先前我们发现，来自小鼠出生后早期脑室下区的神经干和祖细胞（NSPCs）粘附在胶原/透明质酸水凝胶上，而来自成年和胚胎脑的NSPCs则不粘附。方法：为了更详细地研究年轻干细胞的特异性粘附特性，从胚胎、出生后第6天（P6）和成年小鼠大脑中分离的NSPCs在胶原I上培养。结果：出生后早期NSPCs在胶原I上形成paxillin阳性的局点粘附，这种粘附可以被阻断整合素β 1的抗体阻止。此外，我们发现相应的整合素α亚基α 2和α 11水平在出生后阶段最高。差异表达基因的基因本体论分析显示，与成人相比，P6干细胞中参与脉管系统发育和形态发生的转录物表达更高。结论：出生后和成年NSPCs与细胞外基质相互作用的能力不同。一般意义：我们的观察结果表明，早期出生后NSPCs在成年大脑中丢失的特异性粘附特性可以归因于前者表达的整合素亚基，这进一步加深了我们对发育中的神经源性生态位的理解。这篇文章是题为“矩阵介导的细胞行为和特性”的特刊的一部分。(C) 2014年Elsevier B.V.出版
Background: It is becoming increasingly apparent that the extracellular matrix acts as an important regulator of the neural stem niche. Previously we found that neural stem and progenitor cells (NSPCs) derived from the early postnatal subventricular zone of mice adhere to a collagen/hyaluronan hydrogel, whereas NSPCs from the adult and embryonic brain do not.Methods: To examine the specific adhesive properties of young stem cells in more detail, NSPCs isolated from embryonic, postnatal day 6 (P6), and adult mouse brains were cultured on collagen I.Results: Early postnatal NSPCs formed paxillin-positive focal adhesions on collagen I, and these adhesions could be prevented by an antibody that blocked integrin beta 1. Furthermore, we found the corresponding integrin alpha subunits alpha 2 and alpha 11 levels to be highest at the postnatal stage. Gene ontology analysis of differentially expressed genes showed higher expression of transcripts involved in vasculature development and morphogenesis in P6 stem cells, compared to adult.Conclusions: The ability to interact with the extracellular matrix differs between postnatal and adult NSPCs.General significance: Our observations that the specific adhesive properties of early postnatal NSPCs, which are lost in the adult brain, can be ascribed to the integrin subunits expressed by the former furthering our understanding of the developing neurogenic niche. This article is part of a Special Issue entitled Matrix-mediated cell behaviour and properties. (C) 2014 Published by Elsevier B.V.