Dietary Seleno-L-methionine Alters the Microbial Communities and Causes Damage in the Gastrointestinal Tract of Japanese Medaka Oryzias latipes
Dietary Seleno-L-methionine Alters the Microbial Communities and Causes Damage in the Gastrointestinal Tract of Japanese Medaka Oryzias latipes
复制标题
膳食硒-l-蛋氨酸改变微生物群落并对日本青鳉的胃肠道造成损害
DOI：
10.1021/acs.est.1c04533
复制
发表时间：
2021-12-07
影响因子：
11.4
通讯作者：
Xie, Lingtian
中科院分区：
文献类型：
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作者：
Liu, Hongsong;Xiao Li;Xie, Lingtian
饲料中过量硒- l -蛋氨酸（Se-Met）会对生活在硒污染环境中的鱼类产生各种不良影响。然而，关于膳食中过量硒- met对鱼类胃肠道微生物群的影响的数据极度缺乏。本研究以3月龄日本稻幼鱼为研究对象，分别饲喂环境相关浓度为2.90 μ g Se/g dw(对照：(C)、6.69 (L)、11.89 (M)和27.05 (H) μ g Se/g dw的富硒饲粮60 d。采用组织病理学、高通量测序和生物化学等方法，研究了其胃肠道组织学和微生物群落、酶活性以及密切相关基因转录本的变化。结果表明，与L和H处理相比，鱼重降低幅度约为13%。在H处理下，观察到胃肠道绒毛的高度和厚度下降。同时，H处理抑制了d -乳酸水平和二胺氧化酶（DAO）、蛋白酶和脂肪酶的活性。炎症相关基因（IL-1 β和IL-8）的转录本升高，而肠道屏障相关基因（cdh1、ZO-1、ocln和cldn7）的转录本在H处理下被抑制。此外，在属水平上，L处理的α多样性高于对照，饲粮硒- met改变了微生物群落的组成。此外，5个属（红杆菌、Cloacibacterium、Bdellovibrio、Shinella和气单胞菌）在不同处理中表现出最大的丰度变化。本研究表明，过量的硒- met饮食抑制生长，引起胃肠道的病理损伤，并改变水稻微生物群落的组成。
Excess dietary seleno-L-methionine (Se-Met) induces various adverse effects in fish inhabiting the Se-contaminated environments. However, there is an extreme paucity of data on the effects of excess dietary Se-Met on the microbiota in the gastrointestinal (GI) tract in fish. In this study, Japanese medaka Oryzias latipes (three months old) were fed the Se-Met enriched diets at environmentally relevant concentrations: 2.90 (Control: (C), 6.69 (L), 11.89 (M), and 27.05 (H) mu g Se/g dw) for 60 d. Histopathological, high throughput sequencing, and biochemical approaches were used to investigate the alterations in histology and microbial communities of the GI tract, enzymatic activity, and transcripts of closely related genes. The results showed that the fish weight was reduced at similar to 13% from the L and H treatments. Decreased height and thickness of villus in the GI tract were observed in the H treatment. Meanwhile, the level of D-lactate and activity of diamine oxidase (DAO), protease, and lipase were inhibited in the H treatment. The transcripts of the genes related to the inflammation (i.e., IL-1 beta and IL-8) were elevated, while those of the genes related to the intestinal barrier (i.e., cdh1, ZO-1, ocln, and cldn7) were inhibited in the H treatment. In addition, alpha diversity at the genus level was higher in the L treatment than the control, and the composition of the microbial community was altered by dietary Se-Met. Furthermore, 5 genera (Rhodobacter, Cloacibacterium, Bdellovibrio, Shinella, and Aeromonas) exhibited the largest variation in abundance among treatments. This study has demonstrated that excess dietary Se-Met inhibits growth, causes hispathological damage to the GI tract, and alters the composition of the microbial community in Oryzias latipes.