Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry.
Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry.
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DOI：
10.1101/pdb.prot087163
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发表时间：
2016-07-01
影响因子：
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通讯作者：
Waterhouse, Nigel J
中科院分区：
文献类型：
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作者：
Crowley, Lisa C;Scott, Adrian P;Waterhouse, Nigel J
碘化丙啶(PI)是一种与DNA结合的小荧光分子，但不能被动地进入具有完整质膜的细胞。PI摄取和排出可以用来区分死亡细胞和活细胞，在死亡细胞中，无论死亡的机制是什么，质膜都变得可渗透。PI由400到600 nm之间的波长激发，发射600到700 nm之间的光，因此与流动细胞仪中常见的激光和光电探测器兼容。这种PI染色方法可用于对大多数现代研究机构和大学中的细胞死亡进行量化。
Propidium iodide (PI) is a small fluorescent molecule that binds to DNA but cannot passively traverse into cells that possess an intact plasma membrane. PI uptake versus exclusion can be used to discriminate dead cells, in which plasma membranes become permeable regardless of the mechanism of death, from live cells with intact membranes. PI is excited by wavelengths between 400 and 600 nm and emits light between 600 and 700 nm, and is therefore compatible with lasers and photodetectors commonly available in flow cytometers. This protocol for PI staining can be used to quantitate cell death in most modern research facilities and universities.