Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses.
Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses.
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具有流感的工具箱的多模式成像记者病毒。
DOI：
10.3390/v7102873
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发表时间：
2015-10-13
期刊：
影响因子：
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通讯作者：
Mehle A
中科院分区：
文献类型：
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作者：
Tran V;Poole DS;Jeffery JJ;Sheahan TP;Creech D;Yevtodiyenko A;Peat AJ;Francis KP;You S;Mehle A
报告病毒是研究病毒生命周期多个阶段的有用探针。在这里，我们描述了一个扩展的工具箱的荧光和生物发光的甲型流感报告病毒。这些工具的增强效用使得能够对病毒附着、感染和共感染进行动力学研究。感染动物的多模式生物发光和正电子发射断层扫描-计算机断层扫描（PET/CT）成像显示，抗病毒治疗减少了病毒载量、传播和炎症。这些新技术和应用将大大加快流感病毒的体外和体内研究。
Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography–computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.