Proliferation and differentiation of rat calvarial osteoblasts on type I collagen-coated titanium alloy
Proliferation and differentiation of rat calvarial osteoblasts on type I collagen-coated titanium alloy
复制标题
DOI：
10.1002/jbm.1265
复制
发表时间：
2002-03-05
期刊：
影响因子：
--
通讯作者：
Wenzel, KW
中科院分区：
文献类型：
--
作者：
Becker, D;Geissler, U;Wenzel, KW
为了改善钛合金作为种植体材料的骨结合性能，人们对钛合金表面进行了几种尝试。本研究对成骨细胞在I型胶原涂层Ti6Al4V上的增殖、分化和矿化进行了研究。生长在钛合金上的成骨细胞的碱性磷酸酶活性和钙的积累量明显高于生长在聚苯乙烯上的细胞。I型胶原预涂膜对种植体表面的增殖、碱性磷酸酶活性、胶原合成、钙积累以及I型胶原α1、骨桥蛋白、骨钙素、基质金属蛋白酶-2和基质金属蛋白酶-2的表达水平没有广泛的影响。成骨细胞的胶原合成在培养的第4天达到最大值，与植入材料的类型无关。碱性磷酸酶比活力在培养第18天达到最大值。在第22天发现钙的积累和骨钙素的mRNA水平升高。这些结果表明，单纯的胶原涂层不足以促进大鼠颅骨成骨细胞在Ti6Al4V上的分化。(C)2001 John Wiley&Sons，Inc.生物医学杂志59：516-527,2002。
Several attempts have been made to improve osseointegration of titanium alloy as an implant material by modification of its surface. In the present study, proliferation, differentiation, and mineralization of osteoblasts on type I collagen-coated Ti6Al4V were investigated. The activity of alkaline phosphatase and the accumulation of calcium by osteoblasts grown on titanium alloy were significantly higher compared to cells grown on polystyrene. Precoating of the implant surface with type I collagen did not extensively affect proliferation, the activity of alkaline phosphatase, collagen synthesis, calcium accumulation, or the mRNA levels for collagen I alpha1, osteopontin, osteocalcin, MMP-2, and TIMP-2. Maximum collagen synthesis by osteoblasts was observed at day 4 of culture independent of the type of implant material. The specific activity of alkaline phosphatase reached its maximum at day 18 of culture. Accumulation of calcium and elevated mRNA levels for osteocalcin were found at day 22. These results indicate that collagen-coating alone is not sufficient to accelerate differentiation of rat calvarial osteoblasts on Ti6Al4V. (C) 2001 John Wiley & Sons, Inc. J Biomed Mater Res 59: 516-527, 2002.