A FORMULA FOR SCORING HUMAN EMBRYO GROWTH RATES IN IN-VITRO FERTILIZATION ITS VALUE IN PREDICTING PREGNANCY AND IN COMPARISON WITH VISUAL ESTIMATES OF EMBRYO QUALITY
A FORMULA FOR SCORING HUMAN EMBRYO GROWTH RATES IN IN-VITRO FERTILIZATION ITS VALUE IN PREDICTING PREGNANCY AND IN COMPARISON WITH VISUAL ESTIMATES OF EMBRYO QUALITY
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DOI：
10.1007/bf01133388
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
HENNESSEY J F
中科院分区：
文献类型：
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作者：
CUMMINS J M;BREEN T M;HENNESSEY J F
评价了两种用于测量体外胚胎发育的系统。一个是基于从显微镜外观对胚胎质量（EQ）的主观评价的1-4级量表。此外，还建立了胚胎体外生长率的评分公式。胚胎发育评级（EDR）是基于受精后特定阶段观察胚胎的时间与它们预计以假设的“理想”生长速度达到该阶段的时间之间的比率，细胞周期长度为11.9小时。使用该评分系统，“正常”生长的胚胎评分为100。这种方法旨在部分标准化数据，并允许对所有胚胎进行类似的分析，无论观察时间如何。对1539例胚胎置换导致232例临床妊娠的分析表明，EDR和胚胎质量评分在预测成功方面都有价值，临床妊娠最有可能发生在中等至良好EQ评分（2-4）与平均或高于平均生长率（EDR评分从90到129）的组合中。质量差和生长非常缓慢或非常迅速的胚胎在怀孕周期中的代表性不足。这些推断是基于所有移植的胚胎（平均每个移植周期2.73个），并通过对33例单个胚胎置换妊娠和18例两种系统中所有胚胎评分相同的妊娠的分析得到证实。EQ和EDR彼此显著相关，共同为预测妊娠、选择冷冻胚胎和监测体外受精（IVF）计划中的日常表现提供了有价值的指导。
Two systems for measuring embryo development in vitro were evaluated. One was a 1-4 scale based on a subjective evaluation of embryo quality (EQ) from microscopic appearance. In addition, a formula for scoring embryo growth rate in vitro was developed. The embryo development rating (EDR) was based on the ratio between the time at which embryos were observed at a particular stage after insemination and the time at which they would be expected to reach that stage in a hypothetical "ideal" growth rate with a cell cycle length of 11.9 hr. Using this scoring system, "normally" growing embryos scored 100. This approach was aimed at partially normalizing the data and allowed all embryos to be analyzed similarly regardless of the time of observation. Analysis of 1539 embryo replacements resulting in 232 clinical pregnancies showed that both EDR and embryo-quality scores were of value in predicting success, with clinical pregnancy most likely to eventuate from a combination of moderate to good EQ scores (2-4) coupled with average or above-average growth rates (EDR scores from 90 to 129). Poor-quality and very slowly or very rapidly growing embryos were underrepresented in cycles that proceeded to pregnancy. These inferences were based on all embryos transferred (mean, 2.73 per transfer cycle), and they were substantiated by an analysis of 33 pregnancies resulting from replacement of a single embryo and from 18 pregnancies in which all embryos scored the same with both systems. EQ and EDR were significantly associated with each other and together provide a valuable guide in predicting pregnancy, in selecting embryos for freezing, and in monitoring day-to-day performance in the in vitro fertilization (IVF) program.