Preparation of β-1,3-glucanase from scallop mid-gut gland drips and its use for production of novel heterooligosaccharides
Preparation of β-1,3-glucanase from scallop mid-gut gland drips and its use for production of novel heterooligosaccharides
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DOI：
10.1111/j.1444-2906.2008.01632.x
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发表时间：
2008-10-01
影响因子：
1.9
通讯作者：
Ojima, Takao
中科院分区：
文献类型：
--
作者：
Kumagai, Yuya;Inoue, Akira;Ojima, Takao
虾夷扇贝的中肠腺作为渔业废弃物被丢弃在扇贝加工厂中，并且已经进行了各种尝试以将废弃物转化为有价值的资源。本研究以扇贝中肠腺液为原料，研究β-1，3-葡聚糖酶的酶学性质。在北海道县Yubetsu-cho的当地渔业工厂收集中肠腺滴液。β-1，3-葡聚糖酶是通过硫酸铵分级分离，然后在Toyopolysophenyl-650 M和ToyopolysDEAE-650 M上连续层析而从中肠腺滴液中纯化的。扇贝β-1，3-葡聚糖酶在本研究中被命名为PyLam 38，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示分子量约为38 kDa，并且水解了海带多糖（一种来自海带的β-1，3-葡聚糖）。，产生海带二糖和葡萄糖，最适pH和温度分别为6.0和45 ℃。PyLam 38对单糖、醇类和低聚木糖等多种受体底物具有较高的转糖基化活性。因此，发现PyLam 38可用于生产由海带寡糖和各种受体组成的各种新型杂寡糖。
The mid-gut gland of scallop Patinopecten yessoensis has been discarded in scallop processing factories as a fishery waste and various attempts have been made to turn the waste into valuable resources. In the present study, we tried to use mid-gut gland drips from scallop as a source of beta-1,3-glucanase. The mid-gut gland drips were collected in a local fishery factory in Yubetsu-cho, Hokkaido Prefecture. beta-1,3-Glucanase was purified from the mid-gut gland drips by ammonium sulfate fractionation followed by successive chromatography on Toyopearl Phenyl-650M and Toyopearl DEAE-650M. The scallop beta-1,3-glucanase, named PyLam38 in the present study, showed a molecular mass of approximately 38 kDa by sodium dodecylsulfate-polyacrylamide gel electrophoresis, and hydrolyzed laminarin, a beta-1,3-glucan from Laminaria sp., producing laminaribiose and glucose with an optimal pH and temperature of 6.0 and 45 degrees C, respectively. PyLam38 exhibited high transglycosylation activity toward various accepter substrates such as monosaccharides, alcohols and xylooligosaccharides. Thus, PyLam38 was found to be useful for the production of various novel heterooligosaccharides consisting of laminarioligosaccharides and various accepters.