ApoE associated with lipid has a reduced capacity to inhibit β-amyloid fibril formation
ApoE associated with lipid has a reduced capacity to inhibit β-amyloid fibril formation
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DOI：
10.1097/00001756-199810050-00031
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发表时间：
1998-10-05
期刊：
影响因子：
1.7
通讯作者：
Poirier, J
中科院分区：
文献类型：
--
作者：
Beffert, U;Poirier, J
载脂蛋白E(ApoE)和β-淀粉样多肽都存在于与阿尔茨海默病相关的老年斑中。最近的几项研究已经确定载脂蛋白E可以在体外阻止β-淀粉样原纤维(Aβf)的形成。在体内，载脂蛋白E通常与脂源有关。我们发现，重组和纯化的血浆apoE都以剂量依赖的方式抑制Aβf的形成。三种主要的载脂蛋白E亚型在抑制Aβf形成方面是相同的。与含胆固醇脂质体或极低密度脂蛋白相关的APOE对Aβf形成的抑制作用弱于它们的未脂化对应物。这些结果表明，相关脂类的存在降低了载脂蛋白E在体外抑制Aβf形成的能力。(C)1998年，Lippincott Williams&Wilkins。
APOLIPOPROTEIN E (apoE) and the beta-amyloid peptide are both found in the senile plaques associated with Alzheimer's disease. Several studies have recently determined that apoE can prevent beta-amyloid fibril (A beta f) formation in vitro. In vivo, apoE is normally associated with a lipid source. We show that both recombinant and purified plasma apoE inhibit A beta f formation in a dose-dependent manner. The three major apoE isoforms were equipotent at inhibiting A beta f formation. ApoE associated with either cholesterol-containing liposomes or very low density lipoproteins displayed less potent inhibition of A beta f formation than their unlipidated counterparts. These results indicate that the presence of associated lipids reduce the ability of apoE to inhibit the formation of A beta f in vitro. (C) 1998 Lippincott Williams & Wilkins.