Complete Sequence of a blaKPC-Harboring Cointegrate Plasmid Isolated from Escherichia coli
Complete Sequence of a blaKPC-Harboring Cointegrate Plasmid Isolated from Escherichia coli
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DOI：
10.1128/aac.00041-15
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发表时间：
2015-05-01
影响因子：
4.9
通讯作者：
Kreiswirth, Barry N.
中科院分区：
文献类型：
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作者：
Chavda, Kalyan D.;Chen, Liang;Kreiswirth, Barry N.
携带bla（KPC）质粒的水平转移显著促进了肺炎克雷伯菌碳青霉烯酶（KPC）的种间和种内传播。在这里，我们报告了完整的核苷酸序列的bla（KPC）-窝藏IncFIA质粒，pBK 32533，从大肠杆菌。pBK 32533是一个共整合质粒，包含一个与非接合性pBK 30661质粒相同的72-kb序列加上一个含有质粒转移基因的额外170-kb元件。pBK 32533证明bla（KPC）如何通过共整合从非接合质粒中扩散。
Horizontal transfer of bla(KPC)-harboring plasmids contributes significantly to the inter-and intraspecies spread of Klebsiella pneumoniae carbapenemase (KPC). Here we report the complete nucleotide sequence of a bla(KPC)-harboring IncFIA plasmid, pBK32533, from Escherichia coli. pBK32533 is a cointegrate plasmid comprising of a 72-kb sequence identical to that of the non-conjugative pBK30661 plasmid plus an additional 170-kb element that harbors the genes for plasmid transfer. pBK32533 demonstrates how bla(KPC) can be spread from a nonconjugative plasmid through cointegration.