Microdevice for plasma separation from whole human blood using bio-physical and geometrical effects.
Microdevice for plasma separation from whole human blood using bio-physical and geometrical effects.
复制标题
使用生物形态和几何作用的微电位与全人血的血浆分离。
DOI：
10.1038/srep26749
复制
发表时间：
2016-06-09
影响因子：
4.6
通讯作者：
Joshi SS
中科院分区：
文献类型：
--
作者：
Tripathi S;Kumar YV;Agrawal A;Prabhakar A;Joshi SS
在这项研究工作中，我们提出了一种简单而高效的被动式微流控分离装置，用于从纯血液中分离血浆。这种微器件是用传统的光刻技术在聚二甲基硅氧烷的单层上制造的，并已在全血和提高(高达62%)人血的红细胞压积水平上进行了广泛的测试。微器件采用约100 μm的凸起尺寸；这种凸起尺寸确保微器件的无堵塞操作，并且相对容易制造。我们发现，我们的微型设备对未稀释的血液在流速范围内达到了几乎100%的分离效率，流速范围为0.3min到0.5mlm l/ 。通过紫外分光光度法检测蛋白质、hCG(人绒毛膜促性腺激素)激素和随机血糖测试，对从该微装置获得的血浆进行详细的生物学特性测试。此外，还对分离后的血浆进行了流式细胞术研究。这些测试证明回收的血浆质量很高。本文所研制的微装置是对血液在微通道中的流动行为和分离现象进行广泛的实验研究的结果。该微装置结构紧凑、经济有效，特别适用于连续流动作业。
In this research work, we present a simple and efficient passive microfluidic device for plasma separation from pure blood. The microdevice has been fabricated using conventional photolithography technique on a single layer of polydimethylsiloxane, and has been extensively tested on whole blood and enhanced (upto 62%) hematocrit levels of human blood. The microdevice employs elevated dimensions of about 100 μm; such elevated dimensions ensure clog-free operation of the microdevice and is relatively easy to fabricate. We show that our microdevice achieves almost 100% separation efficiency on undiluted blood in the flow rate range of 0.3 to 0.5 ml/min. Detailed biological characterization of the plasma obtained from the microdevice is carried out by testing: proteins by ultra-violet spectrophotometric method, hCG (human chorionic gonadotropin) hormone, and conducting random blood glucose test. Additionally, flow cytometry study has also been carried on the separated plasma. These tests attest to the high quality of plasma recovered. The microdevice developed in this work is an outcome of extensive experimental research on understanding the flow behavior and separation phenomenon of blood in microchannels. The microdevice is compact, economical and effective, and is particularly suited in continuous flow operations.