Alternatively spliced variants of the regucalcin gene in various human normal and tumor tissues
Alternatively spliced variants of the regucalcin gene in various human normal and tumor tissues
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DOI：
10.3892/ijmm.2014.1858
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发表时间：
2014-10-01
影响因子：
5.4
通讯作者：
Yamaguchi, Masayoshi
中科院分区：
文献类型：
--
作者：
Murata, Tomiyasu;Yamaguchi, Masayoshi
Regucalcin作为一种抑制蛋白在多种细胞类型的信号转导中起着关键作用。规则蛋白基因定位于X染色体上，由7个外显子和6个内含子组成。在动物模型中，肝调钙素基因表达的降低被认为在肝细胞癌变的发展中起抑制作用。本研究旨在确定regucalcin基因在人类各种正常组织和肿瘤组织（包括肝、肾、脑和肺组织）中的表达变化。regucalcin mRNA的全长和选择性剪接变体被发现在各种人体组织中表达。该基因在肝细胞癌、肾移行细胞癌、脑恶性脑膜瘤和肺非小细胞癌的肿瘤组织中均被抑制。正常人肝、肾组织中高表达了全长度的regulalcin蛋白；而在肝脏和肾脏肿瘤组织中，其表达被抑制。剪接的变异体蛋白在正常肝脏和肾脏组织中表达，在肿瘤组织中表达减少。在大鼠和小鼠的肝脏和肾脏中未观察到这种替代变异。在人类正常组织和肿瘤组织中发现了规则蛋白基因的选择性剪接变体。
Regucalcin plays a pivotal role as a suppressor protein in signal transduction in various cell types. The regucalcin gene, which is localized on the X chromosome, consists of 7 exons and 6 introns. Decreased liver regucalcin gene expression has been suggested to play a suppressive role in the development of hepatocellular carcinogenesis in animal models. This study was undertaken to determine the changes in regucalcin gene expression in various human normal and tumor tissues, including liver, kidney, brain and lung tissues. The full-length and alternatively spliced variants of regucalcin mRNA were found to be expressed in various human tissues. This expression was suppressed in tumor tissues of hepatocellular carcinoma, kidney transitional cell carcinoma, brain malignant meningioma and lung non-small cell carcinoma. The full-length regucalcin protein was found to be highly expressed in normal human liver and kidney tissues; its expression was suppressed, however, in the liver and kidney tumor tissues. The spliced variant proteins were found to be expressed in the normal liver and kidney tissues, and decreased in the tumor tissues. Such alternative variants were not observed in the liver and kidneys of rats and mice. The alternatively spliced variants of the regucalcin gene were found to be expressed in various human normal and tumor tissues.