Identification of thermophilic species by the amino acid compositions deduced from their genomes
Identification of thermophilic species by the amino acid compositions deduced from their genomes
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DOI：
10.1093/nar/29.7.1608
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发表时间：
2001-04-01
影响因子：
14.9
通讯作者：
Ouzounis, CA
中科院分区：
文献类型：
--
作者：
Kreil, DP;Ouzounis, CA
应用系统聚类和主成分分析方法，对6种嗜热古菌、2种嗜热细菌、17种中温细菌和2种真核生物的全基因组序列进行了氨基酸组成的分析。虽然GC含量具有主导作用，但嗜热物种可以仅通过其全局氨基酸组成来识别。这项研究提出了一个仔细的统计分析影响氨基酸组成的因素，也产生了嗜热物种的平均氨基酸组成的具体功能。此外，我们介绍了第一个例子的“组成树”的物种，不仅考虑到同源蛋白质，而且蛋白质独特的特定物种，我们希望这种简单而新颖的方法是一个有用的额外的工具，在基因组水平上的遗传学研究。
The global amino acid compositions as deduced from the complete genomic sequences of six thermophilic archaea, two thermophilic bacteria, 17 mesophilic bacteria and two eukaryotic species were analysed by hierarchical clustering and principal components analysis, Both methods showed an influence of several factors on amino acid composition. Although GC content has a dominant effect, thermophilic species can be identified by their global amino acid compositions alone. This study presents a careful statistical analysis of factors that affect amino acid composition and also yielded specific features of the average amino acid composition of thermophilic species. Moreover, we introduce the first example of a 'compositional tree' of species that takes into account not only homologous proteins, but also proteins unique to particular species, We expect this simple yet novel approach to be a useful additional tool for the study of phylogeny at the genome level.