Topical formulations with superoxide dismutase: influence of formulation composition on physical stability and enzymatic activity
Topical formulations with superoxide dismutase: influence of formulation composition on physical stability and enzymatic activity
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DOI：
10.1016/s0731-7085(03)00055-4
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发表时间：
2003-04-24
影响因子：
3.4
通讯作者：
Fonseca, MJV
中科院分区：
文献类型：
--
作者：
Di Mambro, VM;Borin, MF;Fonseca, MJV
在三种不同的外用制剂中添加超氧化物歧化酶（SOD），并评估其物理和化学稳定性，以确定维持SOD活性的最稳定制剂。通过在室温、37度和45度保存28天来评估制剂的物理稳定性。每隔7天收集一次样品以评估流变行为。通过测定制剂在室温和45℃下保存75天的酶活性来评价其化学稳定性。该配方表现出假塑性行为，流动指数小于1。流动指数、滞回线和最小表观粘度的初始值无显著差异。单纯乳液和羟乙基纤维素稳定的乳液在第21天粘度下降，温度升高，但SOD的存在没有明显变化。虽然储存时间和温度没有明显变化，但羟乙基纤维素稳定的配方显示SOD活性明显下降。在所研究的配方中加入SOD对其物理稳定性没有影响。简单乳液或用羧甲基稳定的乳液似乎比用羟乙基纤维素稳定的乳液更适合酶添加。(C) 2003 Elsevier Science B.V.版权所有
Three different topical formulations were supplemented with superoxide dismutase (SOD) and evaluated concerning physical and chemical stabilities in order to determine the most stable formulation that would maintain SOD activity. Physical stability was evaluated by storing the formulation at room temperature, and at 37 and 45 degreesC for 28 days. Samples were collected at 7-day intervals for assessment of rheological behavior. Chemical stability was evaluated by the measurement of enzymatic activity in formulations stored at room temperature and at 45 degreesC for 75 days. The formulations showed a pseudoplastic behavior, with a flow index of less than 1. There was no significant difference in the initial values of flow index, hysteresis loop or minimum apparent viscosity. The simple emulsion and the one stabilized with hydroxyethylcellulose showed decreased viscosity by the 21st day and with higher temperature, but no significant changes concerning the presence of SOD. Although there were no significant changes concerning storage time or temperature, the formulation stabilized with hydroxyethylcellulose showed a marked loss of SOD activity. The addition of SOD to the formulations studied did not affect their physical stability. Simple emulsions or emulsions stabilized with carboxypolymethylene seem to be better bases for enzyme addition than emulsion stabilized with hydroxyethylcellulose. (C) 2003 Elsevier Science B.V. All rights reserved.