Neutrophil chemotaxis induced by corneal epithelial cells after herpes simplex virus type 1 infection
Neutrophil chemotaxis induced by corneal epithelial cells after herpes simplex virus type 1 infection
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DOI：
10.1076/ceyr.17.7.687.5164
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发表时间：
1998-07-01
影响因子：
2
通讯作者：
Hayashi, K
中科院分区：
文献类型：
--
作者：
Miyazaki, D;Inoue, Y;Hayashi, K
目的。中性粒细胞侵袭是疱疹性角膜炎发生的主要事件。据报道，角膜细胞的 HSV-1 感染会诱导 IL-8（一种有效的中性粒细胞趋化剂）的合成，而角膜上皮则不会。然而，关于嗜中性粒细胞迁移和HSV-1感染的角膜细胞产生趋化因子之间的细胞关系知之甚少，尤其是在形成眼表面初始屏障的上皮细胞中。我们检查了人角膜上皮细胞和角膜细胞是否可以诱导中性粒细胞趋化以响应 HSV-1 感染。方法。用 SV40 (HCE) 永生化的人角膜上皮细胞和人角膜细胞被 HSV-1 感染。使用改良的博伊登室法测定感染后4、12、24小时收集的培养液的人中性粒细胞趋化性。使用酶联免疫吸附测定 (ELISA) 测量这些上清液中的 IL-8 水平。结果。 HSV-1 MP株感染后HCE和角膜细胞诱导的趋化活性早在感染后4小时达到峰值，然后下降。这些上清液中HSV-1感染的HCE和IL-8水平诱导的趋化活性与感染性病毒滴度平行。它被单克隆抗IL-8抗体抑制。 MP 菌株的 UV 灭活既不能消除趋化活性的诱导，也不能消除受感染 HCE 的 IL-8 分泌。结论。在HSV-1感染的早期，角膜上皮细胞在诱导中性粒细胞趋化性方面发挥着重要作用，该趋化性由IL-8介导。
Purpose. Neutrophil invasion is a primary event in the development of herpetic keratitis. It has been reported that HSV-1 infection of keratocytes induces the synthesis of IL-8, a potent neutrophil chemoattractant, while corneal epithelium does not. Nevertheless, little is known about the cell-elation between neutrophil migration and the production of chemotactic factors by HSV-l-infected corneal cells, especially in epithelial cells which form an initial barrier of the ocular surface. We examined whether human corneal epithelial cells as well as keratocytes could induce neutrophil chemotaxis in response to HSV-1 infection.Methods. Human corneal epithelial cells immortalized with SV40 (HCE) and human keratocytes were infected with HSV-1. The culture fluids collected at 4, 12, 24 h after infection were assayed for human neutrophil chemotaxis using a modified Boyden chamber method. IL-8 levels in these supernatants were measured using enzyme-linked immunosorbent assay (ELISA).Results. The chemotactic activity induced by HCE and keratocytes after MP strain of HSV-1 infection peaked as early as 4 h postinfection, then declined. Chemotactic activity induced by HSV-l-infected HCE and IL-8 levels on these supernatants paralleled with the infectious virus titer. It was inhibited by monoclonal anti-IL-8 antibody. UV-inactivation of MP strain abrogated neither the induction of chemotactic activity nor IL-8 secretion of infected HCE.Conclusions. At the early phase of HSV-1 infection, corneal epithelial cells play an important role in inducing neutrophil chemotaxis, which was mediated by IL-8.