HUMAN PRIMARY PROSTATE TUMOR-CELL LINE, ALVA-31 - A NEW MODEL FOR STUDYING THE HORMONAL-REGULATION OF PROSTATE TUMOR-CELL GROWTH
HUMAN PRIMARY PROSTATE TUMOR-CELL LINE, ALVA-31 - A NEW MODEL FOR STUDYING THE HORMONAL-REGULATION OF PROSTATE TUMOR-CELL GROWTH
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DOI：
10.1002/pros.2990220202
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发表时间：
1993-01-01
期刊：
影响因子：
2.8
通讯作者：
OSTENSON, RC
中科院分区：
文献类型：
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作者：
LOOP, SM;ROZANSKI, TA;OSTENSON, RC
从前列腺切除术中获得的原发肿瘤活检标本中建立了一个新的人前列腺肿瘤细胞系（阿尔瓦-31）。 该细胞系已保持稳定生长超过48个月。体外倍增时间测定为约26小时。染色体数目范围为24-112，在整个连续培养过程中的几个时间点上测试的众数为59。后期传代细胞的核型分析显示约70条人类染色体，8-14个标记，以及两条X染色体，没有Y染色体。前列腺起源证实了前列腺特异性抗原和前列腺酸性磷酸酶的表达，使用特异性抗血清和免疫放射标记技术。前列腺肿瘤异种移植物在完整雄性、去势雄性和雌性无胸腺小鼠中生长;然而，肿瘤生长速率明显取决于血清睾酮水平。
A new human prostate tumor cell line (ALVA-31) has been established from a biopsy specimen of primary tumor obtained during prostatectomy. The cell line has been maintained for more than 48 months in stable growth. The in vitro doubling time was determined to be approximately 26 hr. The chromosome number ranged from 24-112, with a modal number of 59 tested over several time points throughout continuous culture. Karyotypic analysis of late-passaged cells demonstrated approximately 70 human chromosomes, 8-14 markers, and two X chromosomes without a Y chromosome. Prostatic origin was confirmed by the expression of both prostate specific antigen and prostatic acid phosphatase, using specific antisera and immunoradiolabelling techniques. Prostate tumor xenografts were grown in intact male, castrate male, and female athymic mice; however, the rate of tumor growth was clearly dependent upon serum testosterone levels.