Neovascularization of poly(ether ester) block-copolymer scaffolds in vivo:: Long-term investigations using intravital fluorescent microscopy
Neovascularization of poly(ether ester) block-copolymer scaffolds in vivo:: Long-term investigations using intravital fluorescent microscopy
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DOI：
10.1002/jbm.a.20016
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发表时间：
2004-01-01
影响因子：
4.9
通讯作者：
Homann, HH
中科院分区：
文献类型：
--
作者：
Druecke, D;Langer, S;Homann, HH
将不同孔径的聚醚酯嵌段共聚物支架植入balb / c小鼠背侧皮褶腔内。利用活体荧光显微镜，定量分析了这些支架内新生血管的时间过程。植入3组支架，直径5 mm，厚度220 ~ 260 mm， n = 30。评价了不同孔径：小孔径（20-75 μ m）、中孔径（75-212 μ m）和大孔径（250-300 μ m）。于第8、12、16和20天测量周围正常组织、边缘区和支架中心。评估标准微循环参数（血浆泄漏、血管直径、红细胞速度和功能血管密度）。大孔支架的边缘区和中心区功能血管密度（第8天和第12天）明显高于小孔和中等孔支架。这些数据与大孔支架组更大的血管直径和更高的红细胞速度相关。有趣的是，在评估期间，支架边缘的微循环参数恢复到与周围组织相似的值。然而，在植入后20天，支架中心的新生血管仍然活跃。血浆漏量和血管直径在支架中心较高。红细胞速度和功能血管密度比周围组织低50%。总之，小鼠背侧皮肤褶室模型可以长期研究多孔生物医学材料中的血管生长和重塑。聚醚酯嵌段共聚物支架的血管长入速率受孔隙大小的影响，在孔隙最大的支架中血管长入速率最高。该模型生成的数据有助于了解功能性血管的发育和组织向内生长成生物材料。(C) 2003 Wiley期刊有限公司
Poly(ether ester) block-copolymer scaffolds of different pore size were implanted into the dorsal skinfold chamber of balb / c mice. Using intravital fluorescent microscopy, the temporal course of neovascularization into these scaffolds was quantitatively analyzed. Three scaffold groups (diameter, 5 mm; 220-260 thickness, mum; n = 30) were implanted. Different pore sizes were evaluated: small (20-75 mum), medium (75-212 mum) and large pores (250-300 mum). Measurements were performed on days 8, 12, 16, and 20 in the surrounding normal tissue, in the border zone, and in the center of the scaffold. Standard microcirculatory parameters were assessed (plasma leakage, vessel diameter, red blood cell velocity, and functional vessel density). The large-pored scaffolds showed significantly higher functional vessel density in the border zone and in the center (days 8 and 12) compared with the scaffold with the small and medium-sized pores. These data correlated with a larger vessel diameter and a higher red blood cell velocity in the large-pored scaffold group. Interestingly, during the evaluation period the microcirculatory parameters on the edge of the scaffolds returned to values similar to those found in the surrounding tissue. In the center of the scaffold, however, neovascularization was still active 20 days after implantation. Plasma leakage and vessel diameter were higher in the center of the scaffold. Red blood cell velocity and functional vessel density were 50% lower than in the surrounding tissue. In conclusion, the dorsal skinfold chamber model in mice allows long-term study of blood vessel growth and remodeling in porous biomedical materials. The rate of vessel ingrowth into poly(ether ester) block-copolymer scaffolds is influenced by pore size and was highest in the scaffold with the largest pores. The data generated with this model contribute to knowledge about the development of functional vessels and tissue ingrowth into biomaterials. (C) 2003 Wiley Periodicals, Inc.