Physical and Histological Comparison of Hydroxyapatite, Carbonate Apatite, and β-Tricalcium Phosphate Bone Substitutes.
Physical and Histological Comparison of Hydroxyapatite, Carbonate Apatite, and β-Tricalcium Phosphate Bone Substitutes.
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DOI：
10.3390/ma11101993
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发表时间：
2018-10-16
期刊：
影响因子：
--
通讯作者：
Ohe G
中科院分区：
文献类型：
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作者：
Ishikawa K;Miyamoto Y;Tsuchiya A;Hayashi K;Tsuru K;Ohe G
使用杂种犬，比较了三种不同成分的市售人工骨替代品，羟基磷灰石（HAp; Neobone®）、碳酸磷灰石（CO 3Ap; Cytrans®）和β-磷酸三钙（β-TCP; Cerasorb®）的物理特性和对骨的组织反应。Neobone®（HAp）和Cerasorb®（β-TCP）都是多孔的，而Cytrans®（CO 3Ap）是致密的。Neobone®（HAp）、Cytrans®（CO 3Ap）和Cerasorb®（β-TCP）的微晶尺寸和比表面积（SSA）分别为75.4 ± 0.9 nm、30.8 ± 0.8 nm和78.5 ± 7.5 nm，以及0.06 m2/g、18.2 m2/g和1.0 m2/g。这些值与Neobone®（HAp）和Cerasorb®（β-TCP）均为烧结陶瓷，而Cytrans®（CO 3Ap）是在水溶液中制造的事实一致。CO 3Ap（Cytrans®）在模拟Howship's陷窝的pH 5.3溶液中的溶解最快，而β-TCP（Cerasorb®）在pH 7.3生理溶液中的溶解最快。这些结果表明，CO 3Ap在生理条件下是稳定的，并在Howship's陷窝被再吸收。使用混合狗下颌骨骨缺损模型的组织学评价揭示，当在第4周用CO 3Ap（Cytrans®）重建时，从现有骨到骨缺损中心形成新骨。第12周时骨量增加，证实了CO 3Ap（Cytrans®）的再吸收。β-TCP（Cerasorb®）在第4周显示出有限的骨形成。然而，在第12周观察到大量的骨。在这三种骨替代品中，CO 3Ap（Cytrans®）表现出最高水平的新骨形成。这些结果表明，具有与骨相似成分的骨替代物可能具有与骨相似的性质。
Three commercially available artificial bone substitutes with different compositions, hydroxyapatite (HAp; Neobone®), carbonate apatite (CO3Ap; Cytrans®), and β-tricalcium phosphate (β-TCP; Cerasorb®), were compared with respect to their physical properties and tissue response to bone, using hybrid dogs. Both Neobone® (HAp) and Cerasorb® (β-TCP) were porous, whereas Cytrans® (CO3Ap) was dense. Crystallite size and specific surface area (SSA) of Neobone® (HAp), Cytrans® (CO3Ap), and Cerasorb® (β-TCP) were 75.4 ± 0.9 nm, 30.8 ± 0.8 nm, and 78.5 ± 7.5 nm, and 0.06 m2/g, 18.2 m2/g, and 1.0 m2/g, respectively. These values are consistent with the fact that both Neobone® (HAp) and Cerasorb® (β-TCP) are sintered ceramics, whereas Cytrans® (CO3Ap) is fabricated in aqueous solution. Dissolution in pH 5.3 solution mimicking Howship’s lacunae was fastest in CO3Ap (Cytrans®), whereas dissolution in pH 7.3 physiological solution was fastest in β-TCP (Cerasorb®). These results indicated that CO3Ap is stable under physiological conditions and is resorbed at Howship’s lacunae. Histological evaluation using hybrid dog mandible bone defect model revealed that new bone was formed from existing bone to the center of the bone defect when reconstructed with CO3Ap (Cytrans®) at week 4. The amount of bone increased at week 12, and resorption of the CO3Ap (Cytrans®) was confirmed. β-TCP (Cerasorb®) showed limited bone formation at week 4. However, a larger amount of bone was observed at week 12. Among these three bone substitutes, CO3Ap (Cytrans®) demonstrated the highest level of new bone formation. These results indicate the possibility that bone substitutes with compositions similar to that of bone may have properties similar to those of bone.