THE COMPOSITION AND PHYLOGENETIC SIGNIFICANCE OF THE MOUGEOTIA (CHAROPHYCEAE) CELL WALL 1
THE COMPOSITION AND PHYLOGENETIC SIGNIFICANCE OF THE MOUGEOTIA (CHAROPHYCEAE) CELL WALL 1
复制标题
Mougeotia (Charophyceae) 细胞壁的组成和系统发育意义 1
DOI：
--
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
R. M. Malcolm Brown
中科院分区：
文献类型：
--
作者：
A. Hotchkiss;M. Gretz;K. Hicks;R. M. Malcolm Brown
转板藻的两层纤维状细胞壁。琼脂属由63.6%的非纤维素碳水化合物和13.4%的纤维素组成。天然细胞壁中纤维素微纤丝的取向与多网生长假说一致，该假说已被用来解释微纤丝取向从横向（内壁）向轴向（外壁）的转变。分离的细胞壁的单糖分析揭示了十种糖的存在，葡萄糖，木糖和半乳糖最丰富。酸改性的1 N NaOH不溶性残留物级分的甲基化分析表明，其几乎完全由4-连接葡萄糖组成，证实了纤维素的存在。主要半纤维素碳水化合物通过热的1 N NaOH可溶性级分的DEAE Sephacel（Cl−）阴离子交换色谱法进行半纯化。该半纤维素是由4-木糖基主链和2，4-木糖基分支点组成的木聚糖。主要的热水可溶性中性多糖被鉴定为3-连接半乳聚糖。
The two‐layered, fibrillar cell wall of Mougeotia C. Agardh sp. consisted of 63.6% non‐cellulosic carbohydrates and 13.4% cellulose. The orientation of cellulose microfibrils in the native cell wall agrees with the multinet growth hypothesis, which has been employed to explain the shift in microfibril orientation from transverse (inner wall) toward axial (outer wall). Monosaccharide analysis of isolated cell walls revealed the presence of ten sugars with glucose, xylose and galactose most abundant. Methylation analysis of the acid‐modified, 1 N NaOH insoluble residue fraction showed that it was composed almost exclusively of 4‐linked glucose, confirming the presence of cellulose. The major hemicellulosic carbohydrate was semi‐purified by DEAE Sephacel (Cl−) anion‐exchange chromatography of the hot 1 N NaOH soluble fraction. This hemicellulose was a xylan consisting of a 4‐xylosyl backbone and 2,4‐xylosyl branch points. The major hot water soluble neutral polysaccharide was identified as a 3‐linked galactan.