Kinetic analysis of chemotactic peptide receptor modulation
Kinetic analysis of chemotactic peptide receptor modulation
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趋化肽受体调节的动力学分析
DOI：
10.1083/jcb.92.1.34
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
D. Lauffenburger
中科院分区：
文献类型：
--
作者：
S. Zigmond;S. Sullivan;D. Lauffenburger
用氚化肽N-甲酰去甲基亮氨酰苯丙氨酸(FNLLP)观察兔腹膜多形核白细胞趋化肽受体的动态变化。我们使用动力学分析来研究受体丢失(下调)、受体介导肽摄取和受体再循环之间可能的相互关系。我们之前已经证明，与FNLLP孵育的细胞显示出表面可用的受体数量随剂量的减少而减少。这种受体下调在20分钟内完成，然后可用于结合的受体数量保持在平台期水平。即使在下调完成后，肽仍以受体介导的方式继续被摄取。如果肽被去除，受体就会恢复，而不需要蛋白质合成。在这些研究中，我们研究了这些过程的动力学。在此基础上，我们提出高原受体水平是受体内化和回归持续发生的稳态。我们证明，受体介导的多肽摄取率大约等于去除多肽后测得的受体恢复率。此外，受体恢复的速度与表面丢失的受体数量成正比，这表明可能正在发生受体回收。
The dynamics of the chemotactic peptide receptor on rabbit peritoneal polymorphonuclear leucocytes were followed using the tritiated peptide N-formylnorleucylleucylphenylalanine (FNLLP). We have used a kinetic analysis to examine the possible interrelationships between receptor loss (down-regulation), receptor-mediated peptide uptake, and receptor recycling. We have previously demonstrated that cells incubated with FNLLP show a dose-dependent reduction in the number of receptors available on the surface. This receptor down-regulation is complete within 20 min and then the number of receptors available for binding remains at a plateau level. Peptide continues to be taken up in a receptor-mediated manner even after down-regulation is complete. If peptide is removed, receptor recovery occurs and does not require protein synthesis. In these studies we have investigated the kinetics of these processes. On the basis of this analysis, we propose that the plateau receptor level is a steady-state in which receptor internalization and return occur continuously. We demonstrate that the rate of receptor-mediated peptide uptake is approximately equal to the rate of receptor recovery measured after peptide removal. In addition, the rate of receptor recovery is proportional to the number of receptors missing from the surface, suggesting receptor recycling may be occurring.