Dopamine modulates a voltage-gated calcium channel in rat olfactory receptor neurons
Dopamine modulates a voltage-gated calcium channel in rat olfactory receptor neurons
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DOI：
10.1016/s0006-8993(03)02267-4
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发表时间：
2003-04-11
期刊：
影响因子：
2.9
通讯作者：
Toda, K
中科院分区：
文献类型：
--
作者：
Okada, Y;Miyamoto, T;Toda, K
多巴胺D2受体存在于大鼠嗅感受神经元的索马胞体中。采用穿孔式全细胞电压钳技术研究多巴胺对神经元电压门控性钙通道的作用。在10 mM的Ba ~（2+）溶液中，大鼠嗅感受器神经元显示由电压斜坡（167 mV/s）和去极化阶跃脉冲（从保持电位-91mV）引起的内向电流。外源性多巴胺可抑制Ba 2+电流。抑制的IC 50约为1 μ M。Quinpirole（10 μ M，D2多巴胺激动剂）也抑制Ba 2+电流。Quinpirole不影响Ba ~（2+）电流的激活和失活动力学。结果提示，多巴胺对大鼠嗅感受器神经元L-型钙通道的调节是不依赖电压的。(C)2003 Elsevier Science B. V.保留所有权利。
Dopamine D2 receptors exist in the soma of rat olfactory receptor neurons. Actions of dopamine on the voltage-gated Ca2+ channels in the neurons were investigated using the perforated whole-cell voltage-clamp. In 10 mM Ba2+ solution, rat olfactory receptor neurons displayed the inward currents elicited by the voltage ramp (167 mV/s) and depolarizing step pulses from a holding potential of -91 mV The inward Ba2+ currents were greatly reduced by 10 muM nifedipine (L-type Ca2+ channel blocker). The Ba2+ currents were inhibited by the external application of dopamine. The IC50 for the inhibition was about 1 muM. Quinpirole (10 muM, a D2 dopamine agonist) also inhibited the Ba2+ currents. Quinpirole did not affect the activation and inactivation kinetics of the Ba2+ currents. The results suggest that dopamine modulates the L-type Ca2+ channels in rat olfactory receptor neurons via the mechanism independent of voltage. (C) 2003 Elsevier Science B.V. All rights reserved.