Power tools for gene expression and clonal analysis in Drosophila.

Power tools for gene expression and clonal analysis in Drosophila.
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DOI:
10.1038/nmeth.1800
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发表时间:
2011-12-28
期刊:
影响因子:
48
通讯作者:
Desplan, Claude
Desplan, Claude
中科院分区:
生物学1区
文献类型:
--
作者:
Rodriguez, Alberto del Valle;Didiano, Dominic;Desplan, Claude

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果蝇双组分表达系统的发展,最强大的遗传模型之一,允许在特定的细胞群体的基因功能的精确操作。这些表达系统与位点特异性重组方法相结合,也导致了克隆谱系分析新方法的发展。我们提出了一个动手的用户指南的技术和方法,大大提高了分辨率的遗传分析在飞,特别关注他们的应用谱系分析。我们的目的是提供指导和建议,哪些遗传工具是最适合解决不同的发展问题。
The development of two-component expression systems in Drosophila melanogaster, one of the most powerful genetic models, has allowed the precise manipulation of gene function in specific cell populations. These expression systems, in combination with site-specific recombination approaches, have also led to the development of new methods for clonal lineage analysis. We present a hands-on user guide to the techniques and approaches that have greatly increased resolution of genetic analysis in the fly, with a special focus on their application for lineage analysis. Our intention is to provide guidance and suggestions regarding which genetic tools are most suitable for addressing different developmental questions.
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