Power tools for gene expression and clonal analysis in Drosophila.
Power tools for gene expression and clonal analysis in Drosophila.
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DOI:
10.1038/nmeth.1800
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发表时间:
2011-12-28
期刊:
影响因子:
48
通讯作者:
Desplan, Claude
中科院分区:
文献类型:
--
作者:
Rodriguez, Alberto del Valle;Didiano, Dominic;Desplan, Claude
The development of two-component expression systems in Drosophila melanogaster, one of the most powerful genetic models, has allowed the precise manipulation of gene function in specific cell populations. These expression systems, in combination with site-specific recombination approaches, have also led to the development of new methods for clonal lineage analysis. We present a hands-on user guide to the techniques and approaches that have greatly increased resolution of genetic analysis in the fly, with a special focus on their application for lineage analysis. Our intention is to provide guidance and suggestions regarding which genetic tools are most suitable for addressing different developmental questions.
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