Colonization of peanut roots by biofilm-forming Paenibacillus polymyxa initiates biocontrol against crown rot disease
Colonization of peanut roots by biofilm-forming Paenibacillus polymyxa initiates biocontrol against crown rot disease
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DOI：
10.1111/j.1365-2672.2007.03611.x
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发表时间：
2008-04-01
影响因子：
4
通讯作者：
Timmusk, S.
中科院分区：
文献类型：
--
作者：
Haggag, W. M.;Timmusk, S.
目的：研究多粘类芽孢杆菌（Paenibacilluspolymyxa）生物膜形成菌株在防治根腐病中的作用。方法和结果：从黑曲霉抑制土壤中的花生根际分离到两株植物生长促进多粘类芽孢杆菌（Paenibacilluspolymyxa）。在温室和田间条件下测试菌株对花生冠状根腐病病原体的抑制以及在花生根际中的生物膜形成。在模式植物拟南芥根上的定殖和生物被膜形成进一步研究了菌株的固体表面测定。通过田间试验和盆栽试验研究了它们对根腐病的抑制作用。结论：两株菌均能抑制病原菌的生长，但上级生物膜形成剂对冠腐病的防治效果更好。本研究的意义和影响：本研究强调了有效的根际定殖和生物膜形成在生物防治中的重要性。
Aim: To investigate the role of biofilm-forming Paenibacillus polymyxa strains in controlling crown root rot disease.Methods and Results: Two plant growth-promoting P. polymyxa strains were isolated from the peanut rhizosphere, from Aspergillus niger-suppressive soils. The strains were tested, under greenhouse and field conditions for inhibition of the crown root rot pathogen of the peanut, as well as for biofilm formation in the peanut rhizosphere. The strains' colonization and biofilm formation were further studied on roots of the model plant Arabidopsis thaliana and with solid surface assays. Their crown root rot inhibition performance was studied in field and pot experiments. The strains' ability to form biofilms in gnotobiotic and soil systems was studied employing scanning electron microscope.Conclusion: Both strains were able to suppress the pathogen but the superior biofilm former offers significantly better protection against crown rot.Significance and Impact of the Study: The study highlights the importance of efficient rhizosphere colonization and biofilm formation in biocontrol.