Crystallization of selenomethionyl exo-β-1,3-galactanase from the basidiomycete Phanerochaete chrysosporium
Crystallization of selenomethionyl exo-β-1,3-galactanase from the basidiomycete Phanerochaete chrysosporium
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DOI：
10.1107/s1744309109043395
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发表时间：
2009-12-01
影响因子：
0.9
通讯作者：
Samejima, Masahiro
中科院分区：
文献类型：
--
作者：
Ishida, Takuya;Fujimoto, Zui;Samejima, Masahiro
黄孢Phanerochaete chrysosporium的外显- β -1,3-半乳糖酶（pc1,3gal43a）由糖苷水解酶家族43催化结构域和糖结合模块家族35的底物结合结构域组成。它催化-1,3-半乳聚糖的水解，而-1,3-半乳聚糖是阿拉伯半乳聚糖蛋白的主干；c端碳水化合物结合模块家族35结构域通过其对底物的高亲和力增加了β -1,3-半乳聚糖周围酶的局部浓度。为了使用多波长异常色散法测定物相，采用悬挂滴气相扩散法在298 K下结晶硒代甲硫基pc1,3gal43a。x射线吸收光谱证实了晶体中硒的存在。晶体属于P2(1)空间群，衍射分辨率为1.8埃。
Exo-beta-1,3-galactanase from Phanerochaete chrysosporium (Pc1,3Gal43A) consists of a glycoside hydrolase family 43 catalytic domain and a substrate-binding domain that belongs to carbohydrate-binding module family 35. It catalyzes the hydrolysis of beta-1,3-galactan, which is the backbone of the arabinogalactan proteins; the C-terminal carbohydrate-binding module family 35 domain increases the local concentration of the enzyme around beta-1,3-galactan by its high affinity for the substrate. To enable phase determination using the multi-wavelength anomalous dispersion method, selenomethionyl Pc1,3Gal43A was crystallized at 298 K using the hanging-drop vapour-diffusion method. The presence of selenium in the crystals was confirmed from the X-ray absorption spectrum. The crystals belonged to space group P2(1) and diffracted to 1.8 angstrom resolution.