Expression and immunolocalization of aquaporin-6 (Aqp6) in the rat inner ear
Expression and immunolocalization of aquaporin-6 (Aqp6) in the rat inner ear
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DOI：
10.1080/00016480701765691
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发表时间：
2008-01-01
影响因子：
1.4
通讯作者：
Kitano, Hiroya
中科院分区：
文献类型：
--
作者：
Taguchi, Daizo;Takeda, Taizo;Kitano, Hiroya
结论。由于水通道蛋白-6(Aquaporin-6，Aqp6)定位于血管纹、内淋巴囊和前庭细胞内囊泡膜上，Aqp6可能参与了内淋巴液稳态的酸碱代谢和水平衡等生理功能。然而，Aqp6在质膜上没有表达，这表明Aqp6对通过质膜的水通量没有直接作用。目标。目的：探讨Aqp6在大鼠内耳的表达及免疫定位。材料和方法。实验动物为Wistar大鼠。应用逆转录-聚合酶链式反应(RT-PCR)方法检测了Aqp6在大鼠内耳前庭、耳蜗囊和内淋巴囊中的表达，并用免疫荧光显微镜和免疫电子显微镜等免疫组织化学方法研究了Aqp6在大鼠内耳的详细免疫定位。结果。我们获得的新数据表明，Aqp6不仅在耳蜗处表达，而且在内淋巴囊和前庭中也有表达。免疫电子显微镜研究表明，免疫组织化学标记的金广泛分布于血管纹、内淋巴囊和前庭的胞内区，但在细胞膜中未见。
Conclusion. Since aquaporin-6 (Aqp6) protein was located in the membrane of intracellular vesicles of the stria vascularis, endolymphatic sac, and vestibule, Aqp6 might be involved in some distinct physiological function of acid-base metabolism and water balance in endolymphatic fluid homeostasis. However, its lack of expression on the plasma membrane indicates that Aqp6 does not have a direct role in water flux via the plasma membrane. Objective. To evaluate the expression and immunolocalization of Aqp6 in the rat inner ear. Materials and methods. Wistar rats were used. Aqp6 mRNA expression in the rat inner ear was investigated in the vestibulum as well as in the cochlea and endolymphatic sac using the reverse transcription-polymerase chain reaction (RT-PCR) method, and detailed immunolocalization of Aqp6 in the rat inner ear was investigated using immunohistochemical methods including immunofluorescence microscopy and immunoelectron microscopy. Results. We obtained novel data showing that not just Aqp6 mRNA but also Aqp6 protein is expressed in the cochlea, endolymphatic sac, and vestibule. Immunoelectron microscopic studies revealed that the immunolabelled gold was diffusely seen in the intracellular area of the stria vascularis, endolymphatic sac, and vestibule, but never in the plasma membranes.